Abstract
Renewed interest in room-temperature diffraction has been prompted by the desire to observe structural dynamics of proteins as they function. Serial crystallography, an experimental strategy that aggregates small pieces of data from a large uniform pool of crystals, has been demonstrated at synchrotrons and X-ray free-electron lasers. This work utilizes a microfluidic crystallization platform for serial Laue diffraction from macroscopic crystals and proposes that a collection of small slices of Laue data from many individual crystals is a realistic solution to the difficulties in dynamic studies of irreversible biochemical reactions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1975-1982 |
| Number of pages | 8 |
| Journal | Journal of Applied Crystallography |
| Volume | 47 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 1 2014 |
Keywords
- Laue diffraction
- microfluidics
- protein crystallization
- serial crystallography
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)