In situ serial Laue diffraction on a microfluidic crystallization device

Sarah L. Perry, Sudipto Guha, Ashtamurthy S. Pawate, Robert Henning, Irina Kosheleva, Vukica Srajer, Paul J.A. Kenis, Zhong Ren

Research output: Contribution to journalArticlepeer-review

Abstract

Renewed interest in room-temperature diffraction has been prompted by the desire to observe structural dynamics of proteins as they function. Serial crystallography, an experimental strategy that aggregates small pieces of data from a large uniform pool of crystals, has been demonstrated at synchrotrons and X-ray free-electron lasers. This work utilizes a microfluidic crystallization platform for serial Laue diffraction from macroscopic crystals and proposes that a collection of small slices of Laue data from many individual crystals is a realistic solution to the difficulties in dynamic studies of irreversible biochemical reactions.

Original languageEnglish (US)
Pages (from-to)1975-1982
Number of pages8
JournalJournal of Applied Crystallography
Volume47
Issue number6
DOIs
StatePublished - Dec 1 2014

Keywords

  • Laue diffraction
  • microfluidics
  • protein crystallization
  • serial crystallography

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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