In situ localization of barley yellow dwarf virus-PAV 17-kDa protein and nucleic acids in oats

Petra H. Nass, Leslie L. Domier, Birute P. Jakstys, Cleora J. D'Arcy

Research output: Contribution to journalArticlepeer-review


Barley yellow dwarf virus strain PAV (BYDV-PAV) RNA and the 17-kDa protein were localized in BYDV-PAV-infected oat cells using in situ hybridization and in situ immunolocalization assays, respectively. The in situ hybridization assay showed labeling of filamentous material in the nucleus, cytoplasm, and virus-induced vesicles with both sense and antisense nucleic acid probes, suggesting that the filamentous material found in BYDV-PAV-infected cells contains vital RNA. BYDV-PAV negative-strand RNA was detected before virus particles were observed, which indicates that RNA replication is initiated before synthesis of vital coat protein in the cytoplasm. The 17-kDa protein was associated with filamentous material in the cytoplasm, nucleus, and virus-induced vesicles. The labeling densities observed using antibodies against the 17-kDa protein were similar in the nucleus and cytoplasm. No labeling of the 17-kDa protein was observed in plasmodesmata, but filaments in the nuclear pores occasionally were labeled. Since BYDV-PAV RNA and 17-kDa protein colocalized within infected cells, it is possible that single-stranded viral RNA is always associated with the 17-kDa protein in vivo. The 17-kDa protein may be required for vital nucleic acid filaments to traverse the nuclear membrane or other membrane systems.

Original languageEnglish (US)
Pages (from-to)1031-1039
Number of pages9
Issue number10
StatePublished - Oct 1998


  • Electron microscopy
  • Luteovirus

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science


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