In situ interfacial fabrication of an enzymatically cleavable membrane

Dongshin Kim, Jaisree Moorthy, Neil O L Viernes, Jeffrey S. Moore, David J. Beebe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In situ fabrication method utilizing the interfacial reaction of two phase system to realize an enzymatically cleavable membrane was developed using virtual walls created at the interface between a hydrophilic and hydrophobic surface. The hydrophobic surface was generated by the self-assembly of octadecyl trichlorosilane (OTS) on glass. The membrane was formed by incorporating peptide sequences that are cleaved by α-chymotrypsin. An α- chymotrypsin solution was introduced into the hydrophilic side of the channel and after 4 hours the membrane was degraded.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages802-804
Number of pages3
ISBN (Print)0974361119, 9780974361116
StatePublished - Jan 1 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: Oct 9 2005Oct 13 2005

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
CountryUnited States
CityBoston, MA
Period10/9/0510/13/05

Keywords

  • Detection
  • Interfacial fabrication
  • Membrane
  • Microfluidics

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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