In-situ immuno-gold nanoparticle network ELISA biosensors for pathogen detection

Il Hoon Cho, Joseph Irudayaraj

Research output: Contribution to journalArticle

Abstract

Food poisoning microorganisms that contaminate food products and compromise food safety and security have been considered a major health threat and a serious concern for food producers and processors. Developing sensor technologies that are rapid for sensitive and selective detection and quantification of pathogens is a high priority for scientists in academia, state and federal research institutes, and industries. In this work we propose an in-situ immuno-AuNP network-based ELISA biosensor integrated with a sample concentration step based on immuno-magnetic separation to detect pathogenic microorganisms with high sensitivity. The sensor system was optimized by the specific formation of immuno-AuNP network onto the antigenic site present at the outer membrane surface of bacteria and the analytical concept was validated by a microtiter immunoassay. The in-situ network biosensor was able to detect pathogens at extremely low numbers: 3. cells/mL of Escherichia coli O157:H7 and Salmonella typhimurium in buffer and 3. CFU/mL of E. coli O157:H7 and 15. CFU/mL of S. typhimurium in real sample conditions within 2. h of inoculation. The ability to monitor target bacteria with improved analytical sensitivity compared to the current techniques presents a unique opportunity for routine monitoring to improve the safety of foods.

Original languageEnglish (US)
Pages (from-to)70-75
Number of pages6
JournalInternational Journal of Food Microbiology
Volume164
Issue number1
DOIs
StatePublished - Jun 3 2013
Externally publishedYes

Keywords

  • Foodborne pathogens
  • Immuno-gold nanoparticles
  • Immuno-magnetic separation
  • Rapid detection
  • Sensitive

ASJC Scopus subject areas

  • Food Science
  • Microbiology

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