Improving the photosynthetic productivity and light utilization in algal biofuel systems: Metabolic and physiological characterization of a potentially advantageous mutant of chlamydomonas reinhardtii

Y. Zhou, L. C. Schideman, Govindjee, S. I. Rupassara, M. J. Seufferheld

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In this study, we report initial biophysical and biochemical characterization of a spontaneous ‘mutant’ (referred to as ‘IM’) of the green alga Chlamydomonas reinhardtii with several unique attributes that has potential for improving photosynthetic productivity, light utilization efficiency, and, perhaps, even protection against environmental stress. Growth rate experiments showed that under low light intensity (10 μmol photons m−2 s−1), IM showed 36% higher cell density and 25% higher dry cell weight than the wild type cells (WT), while at higher light intensity (640 umol photons m−2 s−1 ), the IM did not show any advantage. Chlorophyll a fluorescence transient measurements and subsequent analysis indicated that IM cells grown at a light intensity of 20 μmol photons m−2 s−1 had a higher light utilization efficiency in comparison to the WT cells. Interestingly, metabolite profiling analysis showed that during the exponential growth phase with both low and high light intensities (10 and 640 umol photons m−2 s−1), the IM cells had higher concentrations than WT cells for several important metabolites that have been previously shown to help protect against environmental stress.

Original languageEnglish (US)
Title of host publicationAdvanced Topics in Science and Technology in China
PublisherSpringer
Pages523-527
Number of pages5
DOIs
StatePublished - Jan 1 2013

Publication series

NameAdvanced Topics in Science and Technology in China
ISSN (Print)1995-6819
ISSN (Electronic)1995-6827

Keywords

  • Chlamydomonas Reinhardtii
  • Fluorescence transient
  • Growth
  • Metabolite profiling
  • Mutant

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Engineering(all)
  • General

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    Zhou, Y., Schideman, L. C., Govindjee, Rupassara, S. I., & Seufferheld, M. J. (2013). Improving the photosynthetic productivity and light utilization in algal biofuel systems: Metabolic and physiological characterization of a potentially advantageous mutant of chlamydomonas reinhardtii. In Advanced Topics in Science and Technology in China (pp. 523-527). (Advanced Topics in Science and Technology in China). Springer. https://doi.org/10.1007/978-3-642-32034-7_111