Improving in vivo maize doubled haploid production efficiency through early detection of false positives

Eunsoo Choe, Christine Hayot Carbonero, Kelly Mulvaney, A. Lane Rayburn, Rita H. Mumm

Research output: Contribution to journalArticlepeer-review


In vivo doubled haploid (DH) technology provides a means of creating new maize inbred lines relatively quickly; however, productivity is limited by false-positive (FP) plants for haploidy and for dihaploidy, which consume resources of space and labour until detected. This work examines the potential for using stomata guard cell length measurement as a means for early detection of FP plants. We found that the true haploid and DH plants could be differentiated from FP and untreated diploid controls as early as Leaf 2 stage by stomata guard cell length measurement. Furthermore, DH plants were distinguishable from haploid and other diploid plants by the Leaf 7 growth stage. Results suggest that, when used together with screening through the anthocyanin colour marker system and flower fertility, stomata guard cell measurement is an easy, non-destructive, early screening method that may lead to a greater efficiency in DH production systems and optimization of resource allocation for space and labour.

Original languageEnglish (US)
Pages (from-to)399-401
Number of pages3
JournalPlant Breeding
Issue number3
StatePublished - Jun 2012


  • Dihaploidy
  • Doubled haploid
  • Maize
  • Quality assurance

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science


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