Improving and repurposing biocatalysts via directed evolution

Carl A. Denard, Hengqian Ren, Huimin Zhao

Research output: Contribution to journalReview articlepeer-review


Over the last two decades, directed evolution has become a staple in protein engineering and ushered in a new era of industrial biocatalysis. Directed evolution has provided the tools to not only improve the activity of known biocatalysts, but also to endow biocatalysts with chemical reactivities not previously encountered in nature. Here we will discuss the recent successes in the quest to enhance thermostability, stereoselectivity and activity of biocatalysts, as well as to create novel enzymes, over the last two years.

Original languageEnglish (US)
Pages (from-to)55-64
Number of pages10
JournalCurrent Opinion in Chemical Biology
StatePublished - Apr 1 2015

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry


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