Improving and repurposing biocatalysts via directed evolution

Carl A. Denard, Hengqian Ren, Huimin Zhao

Research output: Contribution to journalReview article

Abstract

Over the last two decades, directed evolution has become a staple in protein engineering and ushered in a new era of industrial biocatalysis. Directed evolution has provided the tools to not only improve the activity of known biocatalysts, but also to endow biocatalysts with chemical reactivities not previously encountered in nature. Here we will discuss the recent successes in the quest to enhance thermostability, stereoselectivity and activity of biocatalysts, as well as to create novel enzymes, over the last two years.

Original languageEnglish (US)
Pages (from-to)55-64
Number of pages10
JournalCurrent Opinion in Chemical Biology
Volume25
DOIs
StatePublished - Apr 1 2015

Fingerprint

Enzymes
Biocatalysis
Protein Engineering
Stereoselectivity
Chemical reactivity
Proteins

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Improving and repurposing biocatalysts via directed evolution. / Denard, Carl A.; Ren, Hengqian; Zhao, Huimin.

In: Current Opinion in Chemical Biology, Vol. 25, 01.04.2015, p. 55-64.

Research output: Contribution to journalReview article

@article{51de01e252d34ac19579e0e631489dd1,
title = "Improving and repurposing biocatalysts via directed evolution",
abstract = "Over the last two decades, directed evolution has become a staple in protein engineering and ushered in a new era of industrial biocatalysis. Directed evolution has provided the tools to not only improve the activity of known biocatalysts, but also to endow biocatalysts with chemical reactivities not previously encountered in nature. Here we will discuss the recent successes in the quest to enhance thermostability, stereoselectivity and activity of biocatalysts, as well as to create novel enzymes, over the last two years.",
author = "Denard, {Carl A.} and Hengqian Ren and Huimin Zhao",
year = "2015",
month = "4",
day = "1",
doi = "10.1016/j.cbpa.2014.12.036",
language = "English (US)",
volume = "25",
pages = "55--64",
journal = "Current Opinion in Chemical Biology",
issn = "1367-5931",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Improving and repurposing biocatalysts via directed evolution

AU - Denard, Carl A.

AU - Ren, Hengqian

AU - Zhao, Huimin

PY - 2015/4/1

Y1 - 2015/4/1

N2 - Over the last two decades, directed evolution has become a staple in protein engineering and ushered in a new era of industrial biocatalysis. Directed evolution has provided the tools to not only improve the activity of known biocatalysts, but also to endow biocatalysts with chemical reactivities not previously encountered in nature. Here we will discuss the recent successes in the quest to enhance thermostability, stereoselectivity and activity of biocatalysts, as well as to create novel enzymes, over the last two years.

AB - Over the last two decades, directed evolution has become a staple in protein engineering and ushered in a new era of industrial biocatalysis. Directed evolution has provided the tools to not only improve the activity of known biocatalysts, but also to endow biocatalysts with chemical reactivities not previously encountered in nature. Here we will discuss the recent successes in the quest to enhance thermostability, stereoselectivity and activity of biocatalysts, as well as to create novel enzymes, over the last two years.

UR - http://www.scopus.com/inward/record.url?scp=84920913613&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84920913613&partnerID=8YFLogxK

U2 - 10.1016/j.cbpa.2014.12.036

DO - 10.1016/j.cbpa.2014.12.036

M3 - Review article

C2 - 25579451

AN - SCOPUS:84920913613

VL - 25

SP - 55

EP - 64

JO - Current Opinion in Chemical Biology

JF - Current Opinion in Chemical Biology

SN - 1367-5931

ER -