TY - JOUR
T1 - Improving and repurposing biocatalysts via directed evolution
AU - Denard, Carl A.
AU - Ren, Hengqian
AU - Zhao, Huimin
N1 - Publisher Copyright:
© 2014 Elsevier Ltd.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - Over the last two decades, directed evolution has become a staple in protein engineering and ushered in a new era of industrial biocatalysis. Directed evolution has provided the tools to not only improve the activity of known biocatalysts, but also to endow biocatalysts with chemical reactivities not previously encountered in nature. Here we will discuss the recent successes in the quest to enhance thermostability, stereoselectivity and activity of biocatalysts, as well as to create novel enzymes, over the last two years.
AB - Over the last two decades, directed evolution has become a staple in protein engineering and ushered in a new era of industrial biocatalysis. Directed evolution has provided the tools to not only improve the activity of known biocatalysts, but also to endow biocatalysts with chemical reactivities not previously encountered in nature. Here we will discuss the recent successes in the quest to enhance thermostability, stereoselectivity and activity of biocatalysts, as well as to create novel enzymes, over the last two years.
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U2 - 10.1016/j.cbpa.2014.12.036
DO - 10.1016/j.cbpa.2014.12.036
M3 - Review article
C2 - 25579451
AN - SCOPUS:84920913613
SN - 1367-5931
VL - 25
SP - 55
EP - 64
JO - Current Opinion in Chemical Biology
JF - Current Opinion in Chemical Biology
ER -