Improved survival of anchorage-dependent cells in core-shell hydrogel microcapsules via co-encapsulation with cell-friendly microspheres

In Yong Kim, Hyungsoo Choi, Kyekyoon (Kevin) Kim

Research output: Contribution to journalArticle

Abstract

In this study, we investigated the effect of intracapsular environment on the survival of anchorage-dependent cells (ADCs) encapsulated in alginate microcapsules with three different core structures, i.e. liquid, semi-liquid and microsphere-encapsulating semi-liquid core, using NIH 3T3 fibroblasts as an ADC model. For the latter, we fabricated poly (ɛ-caprolactone) microspheres and co-encapsulated them with the cells, to establish cell-substrate interactions in the capsule. The fibroblast cells co-encapsulated with the microspheres exhibited higher survival and growth than those without. This study provides a “proof of concept” for employing microspheres as a cell-friendly surface to establish intracapsular cell-substrate interactions thus prolonging the survival of encapsulated therapeutic ADCs.

Original languageEnglish (US)
Pages (from-to)57-62
Number of pages6
JournalJournal of Microencapsulation
Volume34
Issue number1
DOIs
StatePublished - Jan 2 2017

Keywords

  • Microspheres
  • cell encapsulation
  • co-encapsulation
  • core/shell structure
  • microcapsules

ASJC Scopus subject areas

  • Bioengineering
  • Pharmaceutical Science
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Colloid and Surface Chemistry

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