Abstract
In this study, we investigated the effect of intracapsular environment on the survival of anchorage-dependent cells (ADCs) encapsulated in alginate microcapsules with three different core structures, i.e. liquid, semi-liquid and microsphere-encapsulating semi-liquid core, using NIH 3T3 fibroblasts as an ADC model. For the latter, we fabricated poly (ɛ-caprolactone) microspheres and co-encapsulated them with the cells, to establish cell-substrate interactions in the capsule. The fibroblast cells co-encapsulated with the microspheres exhibited higher survival and growth than those without. This study provides a “proof of concept” for employing microspheres as a cell-friendly surface to establish intracapsular cell-substrate interactions thus prolonging the survival of encapsulated therapeutic ADCs.
Original language | English (US) |
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Pages (from-to) | 57-62 |
Number of pages | 6 |
Journal | Journal of Microencapsulation |
Volume | 34 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2 2017 |
Keywords
- Microspheres
- cell encapsulation
- co-encapsulation
- core/shell structure
- microcapsules
ASJC Scopus subject areas
- Bioengineering
- Pharmaceutical Science
- Physical and Theoretical Chemistry
- Organic Chemistry
- Colloid and Surface Chemistry