Improved purification of acyl carrier protein

Charles O. Rock, John E. Cronan

Research output: Contribution to journalArticlepeer-review


An improved method for the purification of acyl carrier protein from Escherichia coli is described. The method consists of four steps: a 2-propanol extraction, batch adsorption to DEAE-cellulose, ammonium sulfate fractionation, and acid precipitation. The purification can be carried out in a few days and yields between 120 and 150 mg of pure acyl carrier protein per kilogram wet weight of cells.

Original languageEnglish (US)
Pages (from-to)362-364
Number of pages3
JournalAnalytical Biochemistry
Issue number2
StatePublished - Mar 1 1980

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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