Improved purification of acyl carrier protein

Charles O. Rock; John E. Cronan

doi:10.1016/0003-2697(80)90168-2

Improved purification of acyl carrier protein

Research output: Contribution to journal › Article › peer-review

Abstract

An improved method for the purification of acyl carrier protein from Escherichia coli is described. The method consists of four steps: a 2-propanol extraction, batch adsorption to DEAE-cellulose, ammonium sulfate fractionation, and acid precipitation. The purification can be carried out in a few days and yields between 120 and 150 mg of pure acyl carrier protein per kilogram wet weight of cells.

Original language	English (US)
Pages (from-to)	362-364
Number of pages	3
Journal	Analytical Biochemistry
Volume	102
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(80)90168-2
State	Published - Mar 1 1980

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(80)90168-2

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title = "Improved purification of acyl carrier protein",

abstract = "An improved method for the purification of acyl carrier protein from Escherichia coli is described. The method consists of four steps: a 2-propanol extraction, batch adsorption to DEAE-cellulose, ammonium sulfate fractionation, and acid precipitation. The purification can be carried out in a few days and yields between 120 and 150 mg of pure acyl carrier protein per kilogram wet weight of cells.",

author = "Rock, {Charles O.} and Cronan, {John E.}",

note = "Funding Information: This work was supported by the National Institutes of Allergy and Infectious Diseases Grant AI 1056.",

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Y1 - 1980/3/1

N2 - An improved method for the purification of acyl carrier protein from Escherichia coli is described. The method consists of four steps: a 2-propanol extraction, batch adsorption to DEAE-cellulose, ammonium sulfate fractionation, and acid precipitation. The purification can be carried out in a few days and yields between 120 and 150 mg of pure acyl carrier protein per kilogram wet weight of cells.

AB - An improved method for the purification of acyl carrier protein from Escherichia coli is described. The method consists of four steps: a 2-propanol extraction, batch adsorption to DEAE-cellulose, ammonium sulfate fractionation, and acid precipitation. The purification can be carried out in a few days and yields between 120 and 150 mg of pure acyl carrier protein per kilogram wet weight of cells.

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Improved purification of acyl carrier protein

Abstract

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