Abstract
This report describes a new, gentle procedure for rapid and efficient excystation of large numbers of infective sporozoites of Eimeria vermiformis and Eimeria stiedai. Excysted sporozoites are purified using modifications of a previously described ion‐exchange chromatography method. The procedure avoids physical breakage of oocysts and results in greater than 70% recovery of the sporozoites present as sporulated oocysts (i.e. 5–6 sporozoites per sporulated oocyst). The recovered sporozoites are greater than 95% pure and are infective in vivo. We routinely isolate greater than 2 times 108 sporozoites without the use of specialized or expensive equipment.
Original language | English (US) |
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Pages (from-to) | 583-589 |
Number of pages | 7 |
Journal | The Journal of Protozoology |
Volume | 35 |
Issue number | 4 |
DOIs | |
State | Published - Nov 1988 |
Externally published | Yes |
ASJC Scopus subject areas
- Parasitology