Improved Method for High‐Yield Excystation and Purification of Infective Sporozoites of Eimeria spp.

JAMES E. HOSEK, KENNETH S. TODD, MARK S. KUHLENSCHMIDT

Research output: Contribution to journalArticlepeer-review

Abstract

This report describes a new, gentle procedure for rapid and efficient excystation of large numbers of infective sporozoites of Eimeria vermiformis and Eimeria stiedai. Excysted sporozoites are purified using modifications of a previously described ion‐exchange chromatography method. The procedure avoids physical breakage of oocysts and results in greater than 70% recovery of the sporozoites present as sporulated oocysts (i.e. 5–6 sporozoites per sporulated oocyst). The recovered sporozoites are greater than 95% pure and are infective in vivo. We routinely isolate greater than 2 times 108 sporozoites without the use of specialized or expensive equipment.

Original languageEnglish (US)
Pages (from-to)583-589
Number of pages7
JournalThe Journal of Protozoology
Volume35
Issue number4
DOIs
StatePublished - Nov 1988
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology

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