This report describes a new, gentle procedure for rapid and efficient excystation of large numbers of infective sporozoites of Eimeria vermiformis and Eimeria stiedai. Excysted sporozoites are purified using modifications of a previously described ion‐exchange chromatography method. The procedure avoids physical breakage of oocysts and results in greater than 70% recovery of the sporozoites present as sporulated oocysts (i.e. 5–6 sporozoites per sporulated oocyst). The recovered sporozoites are greater than 95% pure and are infective in vivo. We routinely isolate greater than 2 times 108 sporozoites without the use of specialized or expensive equipment.
|Original language||English (US)|
|Number of pages||7|
|Journal||The Journal of Protozoology|
|State||Published - Nov 1988|
ASJC Scopus subject areas