Improved bio-hydrogen production by overexpression of glucose-6-phosphate dehydrogenase and FeFe hydrogenase in Clostridium acetobutylicum

Ye Seung Son; Jong Min Jeon; Do Hyung Kim; Yung Hun Yang; Yong Su Jin; Byung Kwan Cho; Sang Hyoun Kim; Sunil Kumar; Byung Don Lee; Jeong Jun Yoon

doi:10.1016/j.ijhydene.2021.08.222

Improved bio-hydrogen production by overexpression of glucose-6-phosphate dehydrogenase and FeFe hydrogenase in Clostridium acetobutylicum

Ye Seung Son, Jong Min Jeon, Do Hyung Kim, Yung Hun Yang, Yong Su Jin, Byung Kwan Cho, Sang Hyoun Kim, Sunil Kumar, Byung Don Lee, Jeong Jun Yoon

Research output: Contribution to journal › Article › peer-review

Abstract

Clostridium acetobutylicum is an attractive industrial microorganism for biochemical production, but there have been few attempts for bio-hydrogen production based on metabolic engineering. In this study, metabolically engineered C. acetobutylicum carrying glucose-6-phosphate dehydrogenase (zwf) and FeFe hydrogenase (hydA) were constructed as recombinant strains CA-zwf(pIMP-zwf) and CA-hydA(pMTL-hydA), respectively, to improve hydrogen productivity. The results showed that the engineered strains produced 1.15 and 1.39-fold higher hydrogen yield, respectively, than the wild type. Furthermore, when pH and glucose concentration were optimized for the CA-hydA strain, enhanced hydrogen productivity of 25.8% was achieved in 7 L jar scale fermentation. This result provides an insight into the future direction for metabolic engineering of C. acetobutylicum for improved hydrogen production.

Original language	English (US)
Pages (from-to)	36687-36695
Number of pages	9
Journal	International Journal of Hydrogen Energy
Volume	46
Issue number	74
DOIs	https://doi.org/10.1016/j.ijhydene.2021.08.222
State	Accepted/In press - 2021

Keywords

Bio-hydrogen
Clostridium acetobutylicum
FeFe hydrogenase
Glucose-6-phosphate dehydrogenase

ASJC Scopus subject areas

Renewable Energy, Sustainability and the Environment
Fuel Technology
Condensed Matter Physics
Energy Engineering and Power Technology

Online availability

10.1016/j.ijhydene.2021.08.222

Cite this

Son, Y. S., Jeon, J. M., Kim, D. H., Yang, Y. H., Jin, Y. S., Cho, B. K., Kim, S. H., Kumar, S., Lee, B. D., & Yoon, J. J. (Accepted/In press). Improved bio-hydrogen production by overexpression of glucose-6-phosphate dehydrogenase and FeFe hydrogenase in Clostridium acetobutylicum. International Journal of Hydrogen Energy, 46(74), 36687-36695. https://doi.org/10.1016/j.ijhydene.2021.08.222

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title = "Improved bio-hydrogen production by overexpression of glucose-6-phosphate dehydrogenase and FeFe hydrogenase in Clostridium acetobutylicum",

abstract = "Clostridium acetobutylicum is an attractive industrial microorganism for biochemical production, but there have been few attempts for bio-hydrogen production based on metabolic engineering. In this study, metabolically engineered C. acetobutylicum carrying glucose-6-phosphate dehydrogenase (zwf) and FeFe hydrogenase (hydA) were constructed as recombinant strains CA-zwf(pIMP-zwf) and CA-hydA(pMTL-hydA), respectively, to improve hydrogen productivity. The results showed that the engineered strains produced 1.15 and 1.39-fold higher hydrogen yield, respectively, than the wild type. Furthermore, when pH and glucose concentration were optimized for the CA-hydA strain, enhanced hydrogen productivity of 25.8% was achieved in 7 L jar scale fermentation. This result provides an insight into the future direction for metabolic engineering of C. acetobutylicum for improved hydrogen production.",

keywords = "Bio-hydrogen, Clostridium acetobutylicum, FeFe hydrogenase, Glucose-6-phosphate dehydrogenase",

author = "Son, {Ye Seung} and Jeon, {Jong Min} and Kim, {Do Hyung} and Yang, {Yung Hun} and Jin, {Yong Su} and Cho, {Byung Kwan} and Kim, {Sang Hyoun} and Sunil Kumar and Lee, {Byung Don} and Yoon, {Jeong Jun}",

note = "Funding Information: This work was supported by National Research Foundation of Korea grant funded by the Korea government (Ministry of Science and ICT, MSIT) ( NRF-2019M3E6A1103839 ) and the Korea Institute of Energy Technology Evaluation and Planning and the Ministry of Trade, Industry and Energy of the Republic of Korea ( 20188550000540 ). Publisher Copyright: {\textcopyright} 2021 The Author(s)",

year = "2021",

doi = "10.1016/j.ijhydene.2021.08.222",

language = "English (US)",

volume = "46",

pages = "36687--36695",

journal = "International Journal of Hydrogen Energy",

issn = "0360-3199",

publisher = "Elsevier Ltd",

number = "74",

}

TY - JOUR

T1 - Improved bio-hydrogen production by overexpression of glucose-6-phosphate dehydrogenase and FeFe hydrogenase in Clostridium acetobutylicum

AU - Son, Ye Seung

AU - Jeon, Jong Min

AU - Kim, Do Hyung

AU - Yang, Yung Hun

AU - Jin, Yong Su

AU - Cho, Byung Kwan

AU - Kim, Sang Hyoun

AU - Kumar, Sunil

AU - Lee, Byung Don

AU - Yoon, Jeong Jun

N1 - Funding Information: This work was supported by National Research Foundation of Korea grant funded by the Korea government (Ministry of Science and ICT, MSIT) ( NRF-2019M3E6A1103839 ) and the Korea Institute of Energy Technology Evaluation and Planning and the Ministry of Trade, Industry and Energy of the Republic of Korea ( 20188550000540 ). Publisher Copyright: © 2021 The Author(s)

PY - 2021

Y1 - 2021

N2 - Clostridium acetobutylicum is an attractive industrial microorganism for biochemical production, but there have been few attempts for bio-hydrogen production based on metabolic engineering. In this study, metabolically engineered C. acetobutylicum carrying glucose-6-phosphate dehydrogenase (zwf) and FeFe hydrogenase (hydA) were constructed as recombinant strains CA-zwf(pIMP-zwf) and CA-hydA(pMTL-hydA), respectively, to improve hydrogen productivity. The results showed that the engineered strains produced 1.15 and 1.39-fold higher hydrogen yield, respectively, than the wild type. Furthermore, when pH and glucose concentration were optimized for the CA-hydA strain, enhanced hydrogen productivity of 25.8% was achieved in 7 L jar scale fermentation. This result provides an insight into the future direction for metabolic engineering of C. acetobutylicum for improved hydrogen production.

AB - Clostridium acetobutylicum is an attractive industrial microorganism for biochemical production, but there have been few attempts for bio-hydrogen production based on metabolic engineering. In this study, metabolically engineered C. acetobutylicum carrying glucose-6-phosphate dehydrogenase (zwf) and FeFe hydrogenase (hydA) were constructed as recombinant strains CA-zwf(pIMP-zwf) and CA-hydA(pMTL-hydA), respectively, to improve hydrogen productivity. The results showed that the engineered strains produced 1.15 and 1.39-fold higher hydrogen yield, respectively, than the wild type. Furthermore, when pH and glucose concentration were optimized for the CA-hydA strain, enhanced hydrogen productivity of 25.8% was achieved in 7 L jar scale fermentation. This result provides an insight into the future direction for metabolic engineering of C. acetobutylicum for improved hydrogen production.

KW - Bio-hydrogen

KW - Clostridium acetobutylicum

KW - FeFe hydrogenase

KW - Glucose-6-phosphate dehydrogenase

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U2 - 10.1016/j.ijhydene.2021.08.222

DO - 10.1016/j.ijhydene.2021.08.222

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VL - 46

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EP - 36695

JO - International Journal of Hydrogen Energy

JF - International Journal of Hydrogen Energy

IS - 74

ER -

Improved bio-hydrogen production by overexpression of glucose-6-phosphate dehydrogenase and FeFe hydrogenase in Clostridium acetobutylicum

Abstract

Keywords

ASJC Scopus subject areas

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