Improved assay for quantitating adherence of ruminal bacteria to cellulose.

M. A. Rasmussen, B. A. White, R. B. Hespell

Research output: Contribution to journalArticlepeer-review

Abstract

A quantitative technique suitable for the determination of adherence of ruminal bacteria to cellulose was developed. This technique employs adherence of cells to cellulose disks and alleviates the problem of nonspecific cell entrapment within cellulose particles. By using this technique, it was demonstrated that the adherence of Ruminococcus flavefaciens FD1 to cellulose was inhibited by formaldehyde, methylcellulose, and carboxymethyl cellulose. Adherence was unaffected by acid hydrolysates of methylcellulose, glucose, and cellobiose.

Original languageEnglish (US)
Pages (from-to)2089-2091
Number of pages3
JournalApplied and environmental microbiology
Volume55
Issue number8
DOIs
StatePublished - Aug 1989

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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