TY - JOUR
T1 - Impedance microbiology-on-a-chip
T2 - Microfluidic bioprocessor for rapid detection of bacterial metabolism
AU - Gómez-Sjöberg, Rafael
AU - Morisette, Dallas T.
AU - Bashir, Rashid
N1 - Funding Information:
Manuscript received July 23, 2004; revised November 10, 2004. This work was supported by a cooperative agreement with the Agricultural Research Service of the U.S. Department of Agriculture, Project Number 1935-42000-035-00D, through the Center for Food Safety Engineering at Purdue University. Competing Interests Statement: R. Bashir and D. T. Morisette declare competing financial interests. Subject Editor A. J. Ricco.
Funding Information:
The authors would like to thank Prof. M. R. Ladisch and Prof. A. K. Bhunia of Purdue University, and Dr. L. Razouk of BioVitesse, Inc., for useful discussions and helpful suggestions. BioVitesse, Inc., also acknowledges the support from the NSF through an SBIR Phase 1 and Phase 2 Award. They also acknowledge the assistance provided by K. Gray of the Department of Food Science, and the help received from D. Lubelski, T. Miller, B. Crabill, and C. Y. Fong of the microelectronics fabrication facilities at Purdue University, where most of the micro-fabrication process took place. Part of the fabrication process of the bioprocessor was carried out at the Microfabrication Applications Laboratory, University of Illinois, Chicago.
PY - 2005/8
Y1 - 2005/8
N2 - Detection of a few live bacterial cells in many industrial or clinical samples is a very important technological problem. We have developed a microscale technique for concentrating bacterial cells from a dilute sample, by factors on the order of 104 to 105, and detecting their metabolic activity by purely electrical means. The technique was implemented on a silicon-based microfluidic chip where the cells are concentrated and incubated in a chamber with a volume of 400 pL. Concentration and capture are obtained by the use of dielectrophoresis on the bacterial cells, and metabolism detection is achieved by means of impedance measurements of the medium in which the bacteria are incubated. Performing impedance-based detection at the microscale results in drastically reduced detection times for dilute bacterial samples, thanks to the ability to efficiently concentrate and capture the cells in an extremely small volume. Such concentration eliminates the need to amplify the bacterial population by long culture steps. This detection technique can be used for a wide variety of applications.
AB - Detection of a few live bacterial cells in many industrial or clinical samples is a very important technological problem. We have developed a microscale technique for concentrating bacterial cells from a dilute sample, by factors on the order of 104 to 105, and detecting their metabolic activity by purely electrical means. The technique was implemented on a silicon-based microfluidic chip where the cells are concentrated and incubated in a chamber with a volume of 400 pL. Concentration and capture are obtained by the use of dielectrophoresis on the bacterial cells, and metabolism detection is achieved by means of impedance measurements of the medium in which the bacteria are incubated. Performing impedance-based detection at the microscale results in drastically reduced detection times for dilute bacterial samples, thanks to the ability to efficiently concentrate and capture the cells in an extremely small volume. Such concentration eliminates the need to amplify the bacterial population by long culture steps. This detection technique can be used for a wide variety of applications.
KW - Bacterial detection
KW - Bacterial metabolism
KW - Biochip
KW - Impedance microbiology
UR - http://www.scopus.com/inward/record.url?scp=27144548070&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=27144548070&partnerID=8YFLogxK
U2 - 10.1109/JMEMS.2005.845444
DO - 10.1109/JMEMS.2005.845444
M3 - Article
AN - SCOPUS:27144548070
SN - 1057-7157
VL - 14
SP - 829
EP - 838
JO - Journal of Microelectromechanical Systems
JF - Journal of Microelectromechanical Systems
IS - 4
ER -