Impaired mechanical stability, migration and contractile capacity in vimentin deficient fibroblasts

Beate Eckes, Dagmar Dogic, Emma Colucci-Guyon, Ning Wang, Andrew Maniotis, Donald Ingber, Alexandra Merckling, Francina Langa, Monique Aumailley, Annie Delouvée, Victor Koteliansky, Charles Babinet, Thomas Krieg

Research output: Contribution to journalArticle

Abstract

Loss of a vimentin network due to gene disruption created viable mice that did not differ overtly from wild-type littermates. Here, primary fibroblasts derived from vimentin-deficient (-/-) and wild-type (+/+) mouse embryos were cultured, and biological functions were studied in in vitro systems resembling stress situations. Stiffness of -/- fibroblasts was reduced by 40% in comparison to wild-type cells. Vimentin-deficient cells also displayed reduced mechanical stability, motility and directional migration towards different chemo-attractive stimuli. Reorganization of collagen fibrils and contraction of collagen lattices were severely impaired. The spatial organization of focal contact proteins, as well as actin microfilament organization was disturbed. Thus, absence of a vimentin filament network does not impair basic cellular functions needed for growth in culture, but cells are mechanically less stable, and we propose that therefore they are impaired in all functions depending upon mechanical stability.

Original languageEnglish (US)
Pages (from-to)1897-1907
Number of pages11
JournalJournal of Cell Science
Volume111
Issue number13
StatePublished - Aug 21 1998
Externally publishedYes

Keywords

  • Collagen lattice
  • Cytoskeleton
  • In vitro wound healing
  • Mechanotransduction
  • Tensegrity

ASJC Scopus subject areas

  • Cell Biology

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  • Cite this

    Eckes, B., Dogic, D., Colucci-Guyon, E., Wang, N., Maniotis, A., Ingber, D., Merckling, A., Langa, F., Aumailley, M., Delouvée, A., Koteliansky, V., Babinet, C., & Krieg, T. (1998). Impaired mechanical stability, migration and contractile capacity in vimentin deficient fibroblasts. Journal of Cell Science, 111(13), 1897-1907.