Impacts of gold nanoparticle charge and ligand type on surface binding and toxicity to Gram-negative and Gram-positive bacteria

Z. Vivian Feng, Ian L. Gunsolus, Tian A. Qiu, Katie R. Hurley, Lyle H. Nyberg, Hilena Frew, Kyle P. Johnson, Ariane M. Vartanian, Lisa M. Jacob, Samuel E. Lohse, Marco D. Torelli, Robert J. Hamers, Catherine J. Murphy, Christy L. Haynes

Research output: Contribution to journalArticle

Abstract

Although nanomaterials facilitate significant technological advancement in our society, their potential impacts on the environment are yet to be fully understood. In this study, two environmentally relevant bacteria, Shewanella oneidensis and Bacillus subtilis, have been used as model organisms to elucidate the molecular interactions between these bacterial classes and Au nanoparticles (AuNPs) with well-controlled and well-characterized surface chemistries: anionic 3-mercaptopropionic acid (MPA), cationic 3-mercaptopropylamine (MPNH2), and the cationic polyelectrolyte poly(allylamine hydrochloride) (PAH). The data demonstrate that cationic, especially polyelectrolyte-wrapped AuNPs, were more toxic to both the Gram-negative and Gram-positive bacteria. The levels of toxicity observed were closely related to the percentage of cells with AuNPs associated with the cell surface as measured in situ using flow cytometry. The NP concentration-dependent binding profiles were drastically different for the two bacteria strains, suggesting the critical role of bacterial cell surface chemistry in determining nanoparticle association, and thereby, biological impact.

Original languageEnglish (US)
Pages (from-to)5186-5196
Number of pages11
JournalChemical Science
Volume6
Issue number9
DOIs
StatePublished - Jun 15 2015

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ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Feng, Z. V., Gunsolus, I. L., Qiu, T. A., Hurley, K. R., Nyberg, L. H., Frew, H., Johnson, K. P., Vartanian, A. M., Jacob, L. M., Lohse, S. E., Torelli, M. D., Hamers, R. J., Murphy, C. J., & Haynes, C. L. (2015). Impacts of gold nanoparticle charge and ligand type on surface binding and toxicity to Gram-negative and Gram-positive bacteria. Chemical Science, 6(9), 5186-5196. https://doi.org/10.1039/c5sc00792e