Impact of diet on development of bronchial-associated immunity in the neonatal piglet

Shannon C. Thorum, Sarah S. Comstock, Shelly N. Hester, Jill M. Shunk, Marcia Helena Siegel, Sharon M Donovan

Research output: Contribution to journalArticlepeer-review


Bronchial-associated immune development is critically important to protect neonates from respiratory infections. Herein, bronchial-associated immune development in formula-fed and sow-reared pigs is described. Colostrum-fed newborn piglets were fed medicated sow milk replacer formula beginning at 48. h of life or remained with the sow. Blood and tissues were sampled at one-week (d7) and three-weeks (d21) of age. Lymphocyte subpopulations, including T helper 2, cytotoxic T, memory T, and NK cells, in peripheral blood, mediastinal lymph nodes, and thoracic lymph nodes were identified using flow cytometry. Additionally, IL-1β, IL-6, IL-12, TNFα, TGF-β1, TGF-β2, IFNα, IFNβ, and dectin gene expression were analyzed by quantitative RT-PCR. Total IgG, IgM, and IgA concentrations in serum were analyzed. Dietary and developmental effects were observed. This set of baseline measurements provides a framework for future respiratory challenge studies where the effects of diet on the neonate's ability to resist and/or recover from infection can be tested.

Original languageEnglish (US)
Pages (from-to)63-72
Number of pages10
JournalVeterinary Immunology and Immunopathology
Issue number1-2
StatePublished - Jan 15 2013


  • Flow cytometry
  • Immune development
  • Lung
  • Neonate
  • Sus scrofa
  • Swine

ASJC Scopus subject areas

  • Immunology
  • General Veterinary


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