TY - JOUR
T1 - Impact of diet on development of bronchial-associated immunity in the neonatal piglet
AU - Thorum, Shannon C.
AU - Comstock, Sarah S.
AU - Hester, Shelly N.
AU - Shunk, Jill M.
AU - Siegel, Marcia Helena
AU - Donovan, Sharon M
PY - 2013/1/15
Y1 - 2013/1/15
N2 - Bronchial-associated immune development is critically important to protect neonates from respiratory infections. Herein, bronchial-associated immune development in formula-fed and sow-reared pigs is described. Colostrum-fed newborn piglets were fed medicated sow milk replacer formula beginning at 48. h of life or remained with the sow. Blood and tissues were sampled at one-week (d7) and three-weeks (d21) of age. Lymphocyte subpopulations, including T helper 2, cytotoxic T, memory T, and NK cells, in peripheral blood, mediastinal lymph nodes, and thoracic lymph nodes were identified using flow cytometry. Additionally, IL-1β, IL-6, IL-12, TNFα, TGF-β1, TGF-β2, IFNα, IFNβ, and dectin gene expression were analyzed by quantitative RT-PCR. Total IgG, IgM, and IgA concentrations in serum were analyzed. Dietary and developmental effects were observed. This set of baseline measurements provides a framework for future respiratory challenge studies where the effects of diet on the neonate's ability to resist and/or recover from infection can be tested.
AB - Bronchial-associated immune development is critically important to protect neonates from respiratory infections. Herein, bronchial-associated immune development in formula-fed and sow-reared pigs is described. Colostrum-fed newborn piglets were fed medicated sow milk replacer formula beginning at 48. h of life or remained with the sow. Blood and tissues were sampled at one-week (d7) and three-weeks (d21) of age. Lymphocyte subpopulations, including T helper 2, cytotoxic T, memory T, and NK cells, in peripheral blood, mediastinal lymph nodes, and thoracic lymph nodes were identified using flow cytometry. Additionally, IL-1β, IL-6, IL-12, TNFα, TGF-β1, TGF-β2, IFNα, IFNβ, and dectin gene expression were analyzed by quantitative RT-PCR. Total IgG, IgM, and IgA concentrations in serum were analyzed. Dietary and developmental effects were observed. This set of baseline measurements provides a framework for future respiratory challenge studies where the effects of diet on the neonate's ability to resist and/or recover from infection can be tested.
KW - Flow cytometry
KW - Immune development
KW - Lung
KW - Neonate
KW - Sus scrofa
KW - Swine
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UR - http://www.scopus.com/inward/citedby.url?scp=84871691338&partnerID=8YFLogxK
U2 - 10.1016/j.vetimm.2012.10.006
DO - 10.1016/j.vetimm.2012.10.006
M3 - Article
C2 - 23218937
AN - SCOPUS:84871691338
SN - 0165-2427
VL - 151
SP - 63
EP - 72
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
IS - 1-2
ER -