TY - GEN
T1 - Immunosensors for atrazine detection in red wine samples
AU - Valera, Enrique
AU - Rodríguez, Ángel
AU - Ramón-Azcón, Javier
AU - Sanchez, Francisco J.
AU - Marco, M. Pilar
N1 - Funding Information:
*This work was supported by Research Grant GM 139 14 from the National Institutes of Health. t Present address: Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, U.S.A. $ Abbreviations used: Dnp, the 2,4-dinitrophenyl group; NPS, the O-nitrophenylsulfenyl group; CBZ, the carbobenzoxy group; PBS, phosphate buffered saline, 0.15 M NaCl, 0.01 M POAm3.
PY - 2009
Y1 - 2009
N2 - Two novel immunosensors, one impedimetric and other one conductimetric, for atrazine detection in red wine samples have been developed. Impedimetric immunosensor is based on an array of interdigitated \i-electrodes (IDnEs) and bioreagents specifically developed to detect this pesticide. Conductimetric immunosensor incorporates additionally gold nanoparticles. Bioreagents were covalently immobilized on the surface of the electrodes (interdigital space). In both cases the biochemical determination of atrazine is possible without any redox mediator. For the case of the impedimetric immunosensor, the detection method is based on impedimetric measurements (in a wide range of frequencies), whereas in the case of the conductimetric immunosensor the detection method is based on conductimetric measurements (DC measurements).The potential of the impedimetric immunosensor to analyze atrazine in complex sample matrices, such as red wine, have been evaluated. This immunosensor can detect atrazine with limits of detection in the order sub-ppb, far below the maximum residue level (MRL) (50 \ig L"1) established by Euro ean Union (EU) for residues of this herbicide in the wine grapes.
AB - Two novel immunosensors, one impedimetric and other one conductimetric, for atrazine detection in red wine samples have been developed. Impedimetric immunosensor is based on an array of interdigitated \i-electrodes (IDnEs) and bioreagents specifically developed to detect this pesticide. Conductimetric immunosensor incorporates additionally gold nanoparticles. Bioreagents were covalently immobilized on the surface of the electrodes (interdigital space). In both cases the biochemical determination of atrazine is possible without any redox mediator. For the case of the impedimetric immunosensor, the detection method is based on impedimetric measurements (in a wide range of frequencies), whereas in the case of the conductimetric immunosensor the detection method is based on conductimetric measurements (DC measurements).The potential of the impedimetric immunosensor to analyze atrazine in complex sample matrices, such as red wine, have been evaluated. This immunosensor can detect atrazine with limits of detection in the order sub-ppb, far below the maximum residue level (MRL) (50 \ig L"1) established by Euro ean Union (EU) for residues of this herbicide in the wine grapes.
KW - Atrazine
KW - Conductive measurements
KW - Food safety
KW - Immunosensor
KW - Impedance spectroscopy
KW - Interdigitated μ-electrodes
KW - Wine matrix effect
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M3 - Conference contribution
AN - SCOPUS:67650519400
SN - 9789898111647
T3 - BIODEVICES 2009 - Proceedings of the 2nd International Conference on Biomedical Electronics and Devices
SP - 226
EP - 230
BT - BIODEVICES 2009 - Proceedings of the 2nd International Conference on Biomedical Electronics and Devices
T2 - 2nd International Conference on Biomedical Electronics and Devices, BIODEVICES 2009
Y2 - 14 January 2009 through 17 January 2009
ER -