Immunosensors for atrazine detection in red wine samples

Enrique Valera, Ángel Rodríguez, Javier Ramón-Azcón, Francisco J. Sanchez, M. Pilar Marco

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Two novel immunosensors, one impedimetric and other one conductimetric, for atrazine detection in red wine samples have been developed. Impedimetric immunosensor is based on an array of interdigitated \i-electrodes (IDnEs) and bioreagents specifically developed to detect this pesticide. Conductimetric immunosensor incorporates additionally gold nanoparticles. Bioreagents were covalently immobilized on the surface of the electrodes (interdigital space). In both cases the biochemical determination of atrazine is possible without any redox mediator. For the case of the impedimetric immunosensor, the detection method is based on impedimetric measurements (in a wide range of frequencies), whereas in the case of the conductimetric immunosensor the detection method is based on conductimetric measurements (DC measurements).The potential of the impedimetric immunosensor to analyze atrazine in complex sample matrices, such as red wine, have been evaluated. This immunosensor can detect atrazine with limits of detection in the order sub-ppb, far below the maximum residue level (MRL) (50 \ig L"1) established by Euro ean Union (EU) for residues of this herbicide in the wine grapes.

Original languageEnglish (US)
Title of host publicationBIODEVICES 2009 - Proceedings of the 2nd International Conference on Biomedical Electronics and Devices
Pages226-230
Number of pages5
StatePublished - Jul 21 2009
Externally publishedYes
Event2nd International Conference on Biomedical Electronics and Devices, BIODEVICES 2009 - Porto, Portugal
Duration: Jan 14 2009Jan 17 2009

Publication series

NameBIODEVICES 2009 - Proceedings of the 2nd International Conference on Biomedical Electronics and Devices

Conference

Conference2nd International Conference on Biomedical Electronics and Devices, BIODEVICES 2009
CountryPortugal
CityPorto
Period1/14/091/17/09

Keywords

  • Atrazine
  • Conductive measurements
  • Food safety
  • Immunosensor
  • Impedance spectroscopy
  • Interdigitated μ-electrodes
  • Wine matrix effect

ASJC Scopus subject areas

  • Biomedical Engineering
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Immunosensors for atrazine detection in red wine samples'. Together they form a unique fingerprint.

Cite this