ImmunoPET Using engineered antibody fragments: Fluorine-18 labeled diabodies for same-day imaging

Tove Olafsen, Shannon J. Sirk, Sebastian Olma, Clifton K.F. Shen, Anna M. Wu

Research output: Contribution to journalArticlepeer-review

Abstract

Combining the specificity of tumor-targeting antibodies with the sensitivity and quantification offered by positron emission tomography (PET) provides tremendous opportunities for molecular characterization of tumors in vivo. Until recently, significant challenges have been faced when attempting to combine antibodies which show long biological half-lives and positron-emitting radionuclides with comparably short physical half-lives, in particular 18F (half-life, 109 min).A fast and simple microwave-assisted method of generating N-succinimidyl-4-[ 18F]fluorobenzoate has been developed and employed for radiolabeling a small, rapidly targeting HER2-specific engineered antibody fragment, the cysdiabody. Using this tracer, HER2-positive tumor xenografts in mice were detected at 1-4 h post-injection by microPET. This confirms the rapid kinetics of [ 18F]fluorobenzoyl cys-diabody localization, and demonstrates the feasibility of same-day immunoPET imaging. This approach can be broadly applied to antibodies targeting cell surface biomarkers for molecular imaging of tumors and should be highly translatable for clinical use.

Original languageEnglish (US)
Pages (from-to)669-677
Number of pages9
JournalTumor Biology
Volume33
Issue number3
DOIs
StatePublished - Jun 2012
Externally publishedYes

Keywords

  • Antibody fragment
  • HER2 imaging
  • Positron emission tomography
  • Radiochemistry
  • Small animal imaging

ASJC Scopus subject areas

  • Cancer Research

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