Immunological investigation of the distribution of cytochromes related to the two terminal oxidases of Escherichia coli in other gram-negative bacteria

R. G. Kranz, R. B. Gennis

Research output: Contribution to journalArticle

Abstract

Monospecific antibodies were raised against the two terminal oxidase complexes of the aerobic respiratory chain of Escherichia coli. These are the cytochrome d and cytochrome o complexes. The antibodies were used to check for the occurrence of cross-reactive antigens in membrane preparations from a variety of gram-negative bacteria by rocket immunoelectrophoresis and immunoblotting techniques. With these criteria, proteins closely related to the cytochrome d complex of E. coli appeared to be widely distributed. Among the strains containing cytochrome d-related material were Serratia marcescens, Photobacterium phosphoreum, Salmonella typhimurium, Klebsiella pneumoniae, and Azotobacter vinelandii. The data suggest that the d-type terminal oxidase in many of these strains is associated in a complex with b-type and a1-type cytochromes, as has been found to be the case in E. coli, K. pneumoniae and S. typhimurium were also shown to have material cross-reactive to the E. coli cytochrome o complex.

Original languageEnglish (US)
Pages (from-to)709-713
Number of pages5
JournalJournal of bacteriology
Volume161
Issue number2
StatePublished - Jan 1 1985

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Cytochrome d Group
Cytochromes
Gram-Negative Bacteria
Oxidoreductases
Klebsiella pneumoniae
Salmonella typhimurium
Escherichia coli
Cytochromes a1
Photobacterium
Azotobacter vinelandii
Serratia marcescens
Immunoelectrophoresis
Antibodies
Electron Transport
Immunoblotting
Antigens
Membranes
Proteins

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

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title = "Immunological investigation of the distribution of cytochromes related to the two terminal oxidases of Escherichia coli in other gram-negative bacteria",
abstract = "Monospecific antibodies were raised against the two terminal oxidase complexes of the aerobic respiratory chain of Escherichia coli. These are the cytochrome d and cytochrome o complexes. The antibodies were used to check for the occurrence of cross-reactive antigens in membrane preparations from a variety of gram-negative bacteria by rocket immunoelectrophoresis and immunoblotting techniques. With these criteria, proteins closely related to the cytochrome d complex of E. coli appeared to be widely distributed. Among the strains containing cytochrome d-related material were Serratia marcescens, Photobacterium phosphoreum, Salmonella typhimurium, Klebsiella pneumoniae, and Azotobacter vinelandii. The data suggest that the d-type terminal oxidase in many of these strains is associated in a complex with b-type and a1-type cytochromes, as has been found to be the case in E. coli, K. pneumoniae and S. typhimurium were also shown to have material cross-reactive to the E. coli cytochrome o complex.",
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AU - Kranz, R. G.

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N2 - Monospecific antibodies were raised against the two terminal oxidase complexes of the aerobic respiratory chain of Escherichia coli. These are the cytochrome d and cytochrome o complexes. The antibodies were used to check for the occurrence of cross-reactive antigens in membrane preparations from a variety of gram-negative bacteria by rocket immunoelectrophoresis and immunoblotting techniques. With these criteria, proteins closely related to the cytochrome d complex of E. coli appeared to be widely distributed. Among the strains containing cytochrome d-related material were Serratia marcescens, Photobacterium phosphoreum, Salmonella typhimurium, Klebsiella pneumoniae, and Azotobacter vinelandii. The data suggest that the d-type terminal oxidase in many of these strains is associated in a complex with b-type and a1-type cytochromes, as has been found to be the case in E. coli, K. pneumoniae and S. typhimurium were also shown to have material cross-reactive to the E. coli cytochrome o complex.

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