Immunohistochemical detection of Betaine-homocysteine S-methyltransferase in human, pig, and rat liver and kidney

Cassandra V. Delgado-Reyes, Matthew A. Wallig, Timothy A Garrow

Research output: Contribution to journalArticlepeer-review

Abstract

Betaine-homocysteine S-methyltransferase (BHMT) has been shown to be expressed at high levels in the livers of all vertebrate species tested. It has also been shown to be abundant in primate and pig kidney but notably very low in rat kidney and essentially absent from the other major organs of monogastric animals. We recently showed by enzyme activity and Western analysis that pig kidney BHMT was only expressed in the cortex and was absent from the medulla. Using immunohistochemical detection, we report here that in human, pig, and rat kidney, BHMT is expressed in the proximal tubules of the cortex. Immunohistochemical staining for BHMT in human, pig, and rat liver indicate high expression in hepatocytes. The staining patterns are consistent with cytosolic expression in both organs.

Original languageEnglish (US)
Article numberS0003-9861(01)92474-9
Pages (from-to)184-186
Number of pages3
JournalArchives of Biochemistry and Biophysics
Volume393
Issue number1
DOIs
StatePublished - Jan 1 2001

Keywords

  • Betaine
  • Betaine-homocysteine methyltransferase
  • End-stage renal disease
  • Homocysteine

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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