Immunogenicity of ORFV-based vectors expressing the rabies virus glycoprotein in livestock species

Mathias Martins, Lok R. Joshi, Fernando S. Rodrigues, Deniz Anziliero, Rafael Frandoloso, Gerald F. Kutish, Daniel L. Rock, Rudi Weiblen, Eduardo F. Flores, Diego G. Diel

Research output: Contribution to journalArticlepeer-review


The parapoxvirus Orf virus (ORFV) encodes several immunomodulatory proteins (IMPs) that modulate host-innate and pro-inflammatory responses and has been proposed as a vaccine delivery vector for use in animal species. Here we describe the construction and characterization of two recombinant ORFV vectors expressing the rabies virus (RABV) glycoprotein (G). The RABV-G gene was inserted in the ORFV024 or ORFV121 gene loci, which encode for IMPs that are unique to parapoxviruses and inhibit activation of the NF-κB signaling pathway. The immunogenicity of the resultant recombinant viruses (ORFV∆024RABV-G or ORFV∆121RABV-G, respectively) was evaluated in pigs and cattle. Immunization of the target species with ORFV∆024RABV-G and ORFV∆121RABV-G elicited robust neutralizing antibody responses against RABV. Notably, neutralizing antibody titers induced in ORFV∆121RABV-G-immunized pigs and cattle were significantly higher than those detected in ORFV∆024RABV-G-immunized animals, indicating a higher immunogenicity of ORFVΔ121-based vectors in these animal species.

Original languageEnglish (US)
Pages (from-to)229-239
Number of pages11
StatePublished - Nov 2017


  • Glycoprotein
  • ORFV
  • Orf virus
  • Parapoxvirus
  • Rabies
  • Vector

ASJC Scopus subject areas

  • Virology


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