TY - JOUR
T1 - Immunogenicity of nuclear-encoded LTB:ST fusion protein from Escherichia coli expressed in tobacco plants
AU - Rosales-Mendoza, Sergio
AU - Soria-Guerra, Ruth E.
AU - Moreno-Fierros, Leticia
AU - Govea-Alonso, Dania O.
AU - Herrera-Díaz, Areli
AU - Korban, Schuyler S.
AU - Alpuche-Solís, Ángel G.
N1 - Funding Information:
Acknowledgments This research was funded by grants El Potosí estudiantil 2004 (IPICYT), 37048-B, 56980 and 102109 from CO-NACYT and PROMEP 103.5/10/5460. Thanks to Dr. John Clements for providing the LTB protein and Dr. Jim Carrington for supplying the pTRL0027 vector.
PY - 2011/6
Y1 - 2011/6
N2 - Enterotoxigenic Escherichia coli (ETEC) is one of the main causative agents of diarrhea in infants and for travelers. Inclusion of a heat-stable (ST) toxin into vaccine formulations is mandatory as most ETEC strains can produce both heat-labile (LT) and ST enterotoxins. In this study, a genetic fusion gene encoding for an LTB:ST protein has been constructed and transferred into tobacco via Agrobacterium tumefaciens-mediated transformation. Transgenic tobacco plants carrying the LTB:ST gene are then subjected to GM1-ELISA revealing that the LTB:ST has assembled into pentamers and displays antigenic determinants from both LTB and ST. Protein accumulation of up to 0.05% total soluble protein is detected. Subsequently, mucosal and systemic humoral responses are elicited in mice orally dosed with transgenic tobacco leaves. This has suggested that the plant-derived LTB:ST is immunogenic via the oral route. These findings are critical for the development of a plant-based vaccine capable of eliciting broader protection against ETEC and targeting both LTB and ST. Features of this platform in comparison to transplastomic approaches are discussed.
AB - Enterotoxigenic Escherichia coli (ETEC) is one of the main causative agents of diarrhea in infants and for travelers. Inclusion of a heat-stable (ST) toxin into vaccine formulations is mandatory as most ETEC strains can produce both heat-labile (LT) and ST enterotoxins. In this study, a genetic fusion gene encoding for an LTB:ST protein has been constructed and transferred into tobacco via Agrobacterium tumefaciens-mediated transformation. Transgenic tobacco plants carrying the LTB:ST gene are then subjected to GM1-ELISA revealing that the LTB:ST has assembled into pentamers and displays antigenic determinants from both LTB and ST. Protein accumulation of up to 0.05% total soluble protein is detected. Subsequently, mucosal and systemic humoral responses are elicited in mice orally dosed with transgenic tobacco leaves. This has suggested that the plant-derived LTB:ST is immunogenic via the oral route. These findings are critical for the development of a plant-based vaccine capable of eliciting broader protection against ETEC and targeting both LTB and ST. Features of this platform in comparison to transplastomic approaches are discussed.
KW - Broader protective vaccine
KW - Chimeric protein
KW - Oral immunization
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U2 - 10.1007/s00299-011-1023-0
DO - 10.1007/s00299-011-1023-0
M3 - Article
C2 - 21318355
AN - SCOPUS:79955941041
SN - 0721-7714
VL - 30
SP - 1145
EP - 1152
JO - Plant Cell Reports
JF - Plant Cell Reports
IS - 6
ER -