Immature granule neurons from cerebella of different ages exhibit distinct developmental potentials

Lori T. Raetzman, Ruth E. Siegel

Research output: Contribution to journalArticle

Abstract

Cultured cerebellar granule neurons exhibit different developmental potentials in culture dependent on cerebellar age at plating. In cultures prepared at postnatal days (P)2-6, when all granule neurons reside in the external germinal layer (EGL) in vivo, levels of the GABA(A) receptor β2 and γ2 subunit mRNAs are constant. In contrast, in cultures prepared at P8-10, when neurons have begun to migrate into the internal granule cell layer (IGL), the mRNAs increase several-fold in a pattern mimicking that found in vivo. To determine the relationship between neuronal differentiation in culture and potential to express GABA(A) receptor β2 and γ2 subunit transcripts in the mature pattern, neuronal maturity in P6 and P10 cultures was compared. Bromodeoxyuridine labeling studies demonstrated that P10 as well as P6 cultures contained neurons only from the EGL. Moreover, the maturation of cultured P10 and P6 neurons appeared virtually identical. Cells dissociated at both ages expressed mRNAs encoding the EGL markers MATH-1 and TAG-1. The MATH-1 transcript disappeared from cultures maintained 4 days when expression of the GABA(A) receptor α6 subunit, a marker of mature cells, was initiated. Thus, although cultured P6 and P10 granule neurons exhibit the same maturation markers, P10 neurons presumably have been modulated by environmental cues specifying increases in GABA(A) receptor β2 and γ2 subunit expression. This possibility is supported by the finding that extracts of dissociated P10 cells but not P6 cells induce increases in GABA(A) receptor subunit expression in P6 cultures.

Original languageEnglish (US)
Pages (from-to)559-570
Number of pages12
JournalJournal of Neurobiology
Volume38
Issue number4
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

Keywords

  • Differentiation
  • GABA(A) receptor
  • MATH-1
  • Rat cerebellar granule neurons
  • Receptor subunit mRNAs
  • TAG-1

ASJC Scopus subject areas

  • Neuroscience(all)
  • Cellular and Molecular Neuroscience

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