Imaging Method Using CRISPR/dCas9 and Engineered gRNA Scaffolds Can Perturb Replication Timing at the HSPA1 Locus

Keyphrases

• Imaging Methods 100%
• Guide RNA (gRNA) 100%
• Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) 100%
• Replication Timing 100%
• CRISPR-dCas9 100%
• HSPA1 100%
• TetO 66%
• TetR 66%
• DNA Replication Timing 66%
• Living Cells 33%
• Gene Expression 33%
• Enhanced Green Fluorescent Protein (eGFP) 33%
• Mammalian Cells 33%
• DNA-binding Proteins 33%
• DNA Replication 33%
• Tagging Method 33%
• Architectural Research 33%
• TAL Effector 33%
• Genetic Architecture 33%
• Non-repetitive 33%
• Adjacent Genes 33%
• Fluorescent Protein Fusions 33%
• Subnuclear Localization 33%
• Replication Analysis 33%
• Fusion Protein Expression 33%
• Fluorescence Microscopy Imaging 33%
• Gene Replication 33%
• Sister Chromatid Resolution 33%

Biochemistry, Genetics and Molecular Biology

• CRISPR 100%
• Replication Timing 100%
• Gene Expression 20%
• Fluorescence Microscopy 20%
• DNA-binding Protein 20%
• TAL Effector 20%
• Mammalian Cell 20%
• Green Fluorescent Protein 20%
• Fusion Protein 20%
• Protein Expression 20%
• DNA Replication 20%
• Sister Chromatid 20%
• Gene Replication 20%