Imaging lipids with secondary ion mass spectrometry

Mary L. Kraft, Haley A. Klitzing

Research output: Contribution to journalReview articlepeer-review


This review discusses the application of time-of-flight secondary ion mass spectrometry (TOF-SIMS) and magnetic sector SIMS with high lateral resolution performed on a Cameca NanoSIMS 50(L) to imaging lipids. The similarities between the two SIMS approaches and the differences that impart them with complementary strengths are described, and various strategies for sample preparation and to optimize the quality of the SIMS data are presented. Recent reports that demonstrate the new insight into lipid biochemistry that can be acquired with SIMS are also highlighted. This article is part of a Special Issue entitled Tools to study lipid functions.

Original languageEnglish (US)
Pages (from-to)1108-1119
Number of pages12
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Issue number8
StatePublished - Aug 2014


  • Cell membrane
  • Lipid distribution
  • Model membrane
  • NanoSIMS
  • Sample preparation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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