Imaging Higher-order Chromatin Structures in Single Cells Using Stochastic Optical Reconstruction Microscopy

Jianquan Xu, Yang Liu

Research output: Contribution to journalArticlepeer-review

Abstract

Higher-order chromatin organization shaped by epigenetic modifications influence the chromatin environment and subsequently regulate gene expression. Direct visualization of the higher-order chromatin structure at their epigenomic states is of great importance for understanding chromatin compaction and its subsequent effect on gene expression and various cellular processes. With the recent advances in super-resolution microscopy, the higher-order chromatin structure can now be directly visualized in situ down to the scale of ~30 nm. This protocol provides detailed description of super-resolution imaging of higher-order chromatin structure using stochastic optical reconstruction microscopy (STORM). We discussed fluorescence staining methods of DNA and histone proteins and crucial technical factors to obtain high-quality super-resolution images.

Original languageEnglish (US)
Article numbere3160
JournalBio-protocol
Volume9
Issue number3
DOIs
StatePublished - Feb 5 2019
Externally publishedYes

Keywords

  • Epigenetic
  • Higher-order chromatin
  • Histone modification
  • STORM
  • Super-resolution

ASJC Scopus subject areas

  • General Neuroscience
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology
  • Plant Science

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