Imaging heterogeneity of NAD(P)H in individual extracellular vesicles using fluorescence lifetime imaging microscopy (FLIM)

Janet E. Sorrells; Elisabeth M. Martin; Prabuddha Mukherjee; Edita Aksamitiene; Sixian You; Aneesh Alex; Marina Marjanovic; Stephen A. Boppart

doi:10.1364/MICROSCOPY.2020.MW1A.3

Imaging heterogeneity of NAD(P)H in individual extracellular vesicles using fluorescence lifetime imaging microscopy (FLIM)

Janet E. Sorrells, Elisabeth M. Martin, Prabuddha Mukherjee, Edita Aksamitiene, Sixian You, Aneesh Alex, Marina Marjanovic, Stephen A. Boppart

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Individual extracellular vesicles from human epithelial breast cancer cells were imaged using a custom two-photon fluorescence lifetime imaging microscopy system to assess and characterize the heterogeneity of their NAD(P)H content.

Original language	English (US)
Title of host publication	Microscopy Histopathology and Analytics, Microscopy 2020
Publisher	Optica Publishing Group (formerly OSA)
ISBN (Print)	9781943580743
DOIs	https://doi.org/10.1364/MICROSCOPY.2020.MW1A.3
State	Published - 2020
Event	Microscopy Histopathology and Analytics, Microscopy 2020 - Washington, United States Duration: Apr 20 2020 → Apr 23 2020

Publication series

Name	Optics InfoBase Conference Papers
Volume	Part F177-Microscopy-2020
ISSN (Electronic)	2162-2701

Conference

Conference	Microscopy Histopathology and Analytics, Microscopy 2020
Country/Territory	United States
City	Washington
Period	4/20/20 → 4/23/20

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Mechanics of Materials

Online availability

10.1364/MICROSCOPY.2020.MW1A.3

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Sorrells, J. E., Martin, E. M., Mukherjee, P., Aksamitiene, E., You, S., Alex, A., Marjanovic, M., & Boppart, S. A. (2020). Imaging heterogeneity of NAD(P)H in individual extracellular vesicles using fluorescence lifetime imaging microscopy (FLIM). In Microscopy Histopathology and Analytics, Microscopy 2020 (Optics InfoBase Conference Papers; Vol. Part F177-Microscopy-2020). Optica Publishing Group (formerly OSA). https://doi.org/10.1364/MICROSCOPY.2020.MW1A.3

Imaging heterogeneity of NAD(P)H in individual extracellular vesicles using fluorescence lifetime imaging microscopy (FLIM). / Sorrells, Janet E.; Martin, Elisabeth M.; Mukherjee, Prabuddha et al.
Microscopy Histopathology and Analytics, Microscopy 2020. Optica Publishing Group (formerly OSA), 2020. (Optics InfoBase Conference Papers; Vol. Part F177-Microscopy-2020).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Sorrells, JE, Martin, EM, Mukherjee, P, Aksamitiene, E, You, S, Alex, A, Marjanovic, M & Boppart, SA 2020, Imaging heterogeneity of NAD(P)H in individual extracellular vesicles using fluorescence lifetime imaging microscopy (FLIM). in Microscopy Histopathology and Analytics, Microscopy 2020. Optics InfoBase Conference Papers, vol. Part F177-Microscopy-2020, Optica Publishing Group (formerly OSA), Microscopy Histopathology and Analytics, Microscopy 2020, Washington, United States, 4/20/20. https://doi.org/10.1364/MICROSCOPY.2020.MW1A.3

Sorrells JE, Martin EM, Mukherjee P, Aksamitiene E, You S, Alex A et al. Imaging heterogeneity of NAD(P)H in individual extracellular vesicles using fluorescence lifetime imaging microscopy (FLIM). In Microscopy Histopathology and Analytics, Microscopy 2020. Optica Publishing Group (formerly OSA). 2020. (Optics InfoBase Conference Papers). doi: 10.1364/MICROSCOPY.2020.MW1A.3

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title = "Imaging heterogeneity of NAD(P)H in individual extracellular vesicles using fluorescence lifetime imaging microscopy (FLIM)",

abstract = "Individual extracellular vesicles from human epithelial breast cancer cells were imaged using a custom two-photon fluorescence lifetime imaging microscopy system to assess and characterize the heterogeneity of their NAD(P)H content.",

author = "Sorrells, {Janet E.} and Martin, {Elisabeth M.} and Prabuddha Mukherjee and Edita Aksamitiene and Sixian You and Aneesh Alex and Marina Marjanovic and Boppart, {Stephen A.}",

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AU - Mukherjee, Prabuddha

AU - Aksamitiene, Edita

AU - You, Sixian

AU - Alex, Aneesh

AU - Marjanovic, Marina

AU - Boppart, Stephen A.

PY - 2020

Y1 - 2020

N2 - Individual extracellular vesicles from human epithelial breast cancer cells were imaged using a custom two-photon fluorescence lifetime imaging microscopy system to assess and characterize the heterogeneity of their NAD(P)H content.

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DO - 10.1364/MICROSCOPY.2020.MW1A.3

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AN - SCOPUS:85091401095

SN - 9781943580743

T3 - Optics InfoBase Conference Papers

BT - Microscopy Histopathology and Analytics, Microscopy 2020

PB - Optica Publishing Group (formerly OSA)

T2 - Microscopy Histopathology and Analytics, Microscopy 2020

Y2 - 20 April 2020 through 23 April 2020

ER -

Imaging heterogeneity of NAD(P)H in individual extracellular vesicles using fluorescence lifetime imaging microscopy (FLIM)

Abstract

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