Imaging collagen properties in the uterosacral ligaments of women with pelvic organ prolapse using spatial light interference microscopy (SLIM)

Chenfei Hu, Melissa Santi, Oluwatobi Adelaja, Andre Kajdacsy-Balla, Gabriel Popescu, William Kobak

Research output: Contribution to journalArticle

Abstract

We studied collagen fiber organization in tissue affected by pelvic organ prolapse (POP) and compared it to asymptomatic controls. Both the control and POP tissue biopsies were prepared and measured by a highly sensitive quantitative phase imaging (QPI) system, called spatial light interference microscopy (SLIM). Combined with automatic image processing, this modality provides quantitative, high-throughput assessment of fiber morphology. We found the fiber orientation in prolapsed specimens is less homogeneous, indicating an abnormal organization of collagen in the extracellular matrix (ECM).

Original languageEnglish (US)
Article number72
JournalFrontiers in Physics
Volume7
Issue numberMay
DOIs
StatePublished - 2019

Keywords

  • Fiber extraction
  • Imaging processing
  • Pelvic organ prolapse
  • Quantitative Phase Imaging (QPI)
  • SLIM
  • Tissue

ASJC Scopus subject areas

  • Biophysics
  • Materials Science (miscellaneous)
  • Mathematical Physics
  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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