IL-10 promotes survival of microglia without activating Akt

Klemen Strle, Jian Hua Zhou, Suzanne R. Broussard, Homer D. Venters, Rodney W. Johnson, Gregory G. Freund, Robert Dantzer, Keith W. Kelley

Research output: Contribution to journalArticle

Abstract

IL-10 is an anti-inflammatory cytokine that has recently been shown to promote survival of neurons and glia. Here we establish that IL-10 induces phosphorylation of Stat3 on Tyr705 and serves as a survival factor for N13 microglial cells. Recombinant IL-10 (10 ng/ml) decreases growth factor withdrawal-induced apoptosis by 50%, as assessed by TUNEL. In contrast to IL-10, IGF-I increases enzymatic activity of PI 3-kinase and causes phosphorylation on serine473 of Akt but does not prevent microglial apoptosis. These data establish that IL-10 activates Stat3 and inhibits the mitochondrial pathway of cell death without activating the Akt cell survival pathway.

Original languageEnglish (US)
Pages (from-to)9-19
Number of pages11
JournalJournal of Neuroimmunology
Volume122
Issue number1-2
DOIs
StatePublished - 2002

Keywords

  • Akt
  • Apoptosis
  • IGF-I
  • IL-10
  • Phosphatidylinositol 3′ -kinase
  • Stat3

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

Fingerprint Dive into the research topics of 'IL-10 promotes survival of microglia without activating Akt'. Together they form a unique fingerprint.

  • Cite this

    Strle, K., Zhou, J. H., Broussard, S. R., Venters, H. D., Johnson, R. W., Freund, G. G., Dantzer, R., & Kelley, K. W. (2002). IL-10 promotes survival of microglia without activating Akt. Journal of Neuroimmunology, 122(1-2), 9-19. https://doi.org/10.1016/S0165-5728(01)00444-1