IL-10 promotes survival of microglia without activating Akt

Klemen Strle, Jian Hua Zhou, Suzanne R. Broussard, Homer D. Venters, Rodney W. Johnson, Gregory G Freund, Robert Dantzer, Keith W. Kelley

Research output: Contribution to journalArticlepeer-review


IL-10 is an anti-inflammatory cytokine that has recently been shown to promote survival of neurons and glia. Here we establish that IL-10 induces phosphorylation of Stat3 on Tyr705 and serves as a survival factor for N13 microglial cells. Recombinant IL-10 (10 ng/ml) decreases growth factor withdrawal-induced apoptosis by 50%, as assessed by TUNEL. In contrast to IL-10, IGF-I increases enzymatic activity of PI 3-kinase and causes phosphorylation on serine473 of Akt but does not prevent microglial apoptosis. These data establish that IL-10 activates Stat3 and inhibits the mitochondrial pathway of cell death without activating the Akt cell survival pathway.

Original languageEnglish (US)
Pages (from-to)9-19
Number of pages11
JournalJournal of Neuroimmunology
Issue number1-2
StatePublished - 2002


  • Akt
  • Apoptosis
  • IGF-I
  • IL-10
  • Phosphatidylinositol 3′ -kinase
  • Stat3

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology


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