Abstract
IL-10 is an anti-inflammatory cytokine that has recently been shown to promote survival of neurons and glia. Here we establish that IL-10 induces phosphorylation of Stat3 on Tyr705 and serves as a survival factor for N13 microglial cells. Recombinant IL-10 (10 ng/ml) decreases growth factor withdrawal-induced apoptosis by 50%, as assessed by TUNEL. In contrast to IL-10, IGF-I increases enzymatic activity of PI 3-kinase and causes phosphorylation on serine473 of Akt but does not prevent microglial apoptosis. These data establish that IL-10 activates Stat3 and inhibits the mitochondrial pathway of cell death without activating the Akt cell survival pathway.
Original language | English (US) |
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Pages (from-to) | 9-19 |
Number of pages | 11 |
Journal | Journal of Neuroimmunology |
Volume | 122 |
Issue number | 1-2 |
DOIs | |
State | Published - 2002 |
Keywords
- Akt
- Apoptosis
- IGF-I
- IL-10
- Phosphatidylinositol 3′ -kinase
- Stat3
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Neurology
- Clinical Neurology