Identification of the sex-determining region in flathead grey mullet (Mugil cephalus)

L. Dor, A. Shirak, H. Rosenfeld, I. M. Ashkenazi, Mark Robert Band, A. Korol, Y. Ronin, E. Seroussi, J. I. Weller, M. Ron

Research output: Contribution to journalArticle

Abstract

Elucidation of the sex-determination mechanism in flathead grey mullet (Mugil cephalus) is required to exploit its economic potential by production of genetically determined monosex populations and application of hormonal treatment to parents rather than to the marketed progeny. Our objective was to construct a first-generation linkage map of the M. cephalus in order to identify the sex-determining region and sex-determination system. Deep-sequencing data of a single male was assembled and aligned to the genome of Nile tilapia (Oreochromis niloticus). A total 245 M. cephalus microsatellite markers were designed, spanning the syntenic tilapia genome assembly at intervals of 10 Mb. In the mapping family of full-sib progeny, 156 segregating markers were used to construct a first-generation linkage map of 24 linkage groups (LGs), corresponding to the number of chromosomes. The linkage map spanned approximately 1200 cM with an average inter-marker distance of 10.6 cM. Markers segregating on LG9 in two independent mapping families showed nearly complete concordance with gender (R2 = 0.95). The sex determining locus was fine mapped within an interval of 8.6 cM on LG9. The sex of offspring was determined only by the alleles transmitted from the father, thus indicating an XY sex-determination system.

Original languageEnglish (US)
Pages (from-to)698-707
Number of pages10
JournalAnimal genetics
Volume47
Issue number6
DOIs
StatePublished - Dec 1 2016

Fingerprint

Smegmamorpha
Mugil cephalus
gender
Cichlids
chromosome mapping
Oreochromis niloticus
Genome
Tilapia
High-Throughput Nucleotide Sequencing
Tilapia (Cichlidae)
genome assembly
Fathers
Microsatellite Repeats
fathers
linkage groups
Chromosomes
Parents
Alleles
Economics
microsatellite repeats

Keywords

  • comparative mapping
  • fine mapping
  • linkage map
  • microsatellite markers
  • sex-determining system

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics

Cite this

Dor, L., Shirak, A., Rosenfeld, H., Ashkenazi, I. M., Band, M. R., Korol, A., ... Ron, M. (2016). Identification of the sex-determining region in flathead grey mullet (Mugil cephalus). Animal genetics, 47(6), 698-707. https://doi.org/10.1111/age.12486

Identification of the sex-determining region in flathead grey mullet (Mugil cephalus). / Dor, L.; Shirak, A.; Rosenfeld, H.; Ashkenazi, I. M.; Band, Mark Robert; Korol, A.; Ronin, Y.; Seroussi, E.; Weller, J. I.; Ron, M.

In: Animal genetics, Vol. 47, No. 6, 01.12.2016, p. 698-707.

Research output: Contribution to journalArticle

Dor, L, Shirak, A, Rosenfeld, H, Ashkenazi, IM, Band, MR, Korol, A, Ronin, Y, Seroussi, E, Weller, JI & Ron, M 2016, 'Identification of the sex-determining region in flathead grey mullet (Mugil cephalus)', Animal genetics, vol. 47, no. 6, pp. 698-707. https://doi.org/10.1111/age.12486
Dor, L. ; Shirak, A. ; Rosenfeld, H. ; Ashkenazi, I. M. ; Band, Mark Robert ; Korol, A. ; Ronin, Y. ; Seroussi, E. ; Weller, J. I. ; Ron, M. / Identification of the sex-determining region in flathead grey mullet (Mugil cephalus). In: Animal genetics. 2016 ; Vol. 47, No. 6. pp. 698-707.
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