Identification of the G-protein α-subunit encoded by αo2 cDNA as a 39 kDa pertussis toxin substrate

K. Spicher, F. J. Klinz, U. Rudolph, J. Codina, L. Birnbaumer, G. Schultz, W. Rosenthal

Research output: Contribution to journalArticlepeer-review

Abstract

A novel form of the Go α-subunit (αo2) has been identified by molecular cloning (Hsu et al., J. Biol. Chem. 265, 11220-11226, 1990). An antibody was generated against a synthetic peptide corresponding to a region of the protein encoded by αo2 cDNA. The antibody reacted with an apparently single 39 kDa protein in membrane preparations of rodent brain and with a 39 kDa pertussis toxin substrate in membranes of rodent neuroendocrine and pituitary cells. A previously produced antibody raised against a region common to proteins encoded by αo2 cDNA and the previous cloned αo1 cDNA (Itoh et al., Proc. Natl. Acad. Sci. USA 83, 3776-3780, 1986) recognized proteins of 39 and 40 kDa in preparations of bovine, porcine and rodent brain and pertussis toxin substrates of 39 and 40 kDa in membranes of rodent neuroendocrine and pituitary cells. We conclude that the 39 kDa Go α subunit is encoded by αo2 cDNA.

Original languageEnglish (US)
Pages (from-to)473-479
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume175
Issue number2
DOIs
StatePublished - Mar 15 1991
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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