TY - JOUR
T1 - Identification of the first riboflavin catabolic gene cluster isolated from microbacterium maritypicum G10
AU - Xu, Hui
AU - Chakrabarty, Yindrila
AU - Philmus, Benjamin
AU - Mehta, Angad P.
AU - Bhandari, Dhananjay
AU - Hohmann, Hans Peter
AU - Begley, Tadhg P.
N1 - Publisher Copyright:
© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.
PY - 2016/11/4
Y1 - 2016/11/4
N2 - Riboflavinisa common cofactor, and its biosynthetic pathway is well characterized. However, its catabolic pathway, despite intriguing hints in a few distinct organisms, has never been established. This article describes the isolation of a Microbacterium maritypicum riboflavin catabolic strain, and the cloning of the riboflavin catabolic genes. RcaA, RcaB, RcaD, and RcaE were overexpressed and biochemically characterized as riboflavin kinase, riboflavin reductase, ribokinase, and riboflavin hydrolase, respectively. Based on these activities, a pathway for ribo-flavin catabolism is proposed.
AB - Riboflavinisa common cofactor, and its biosynthetic pathway is well characterized. However, its catabolic pathway, despite intriguing hints in a few distinct organisms, has never been established. This article describes the isolation of a Microbacterium maritypicum riboflavin catabolic strain, and the cloning of the riboflavin catabolic genes. RcaA, RcaB, RcaD, and RcaE were overexpressed and biochemically characterized as riboflavin kinase, riboflavin reductase, ribokinase, and riboflavin hydrolase, respectively. Based on these activities, a pathway for ribo-flavin catabolism is proposed.
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U2 - 10.1074/jbc.M116.729871
DO - 10.1074/jbc.M116.729871
M3 - Article
C2 - 27590337
AN - SCOPUS:84994314466
SN - 0021-9258
VL - 291
SP - 23506
EP - 23515
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 45
ER -