TY - JOUR
T1 - Identification of the aggregation-sex pheromone of Plagionotus arcuatus ssp. arcuatus (Coleoptera: Cerambycidae) from two geographically separated European populations
AU - Imrei, Zoltán
AU - Molander, Mikael A.
AU - Winde, Inis B.
AU - Lohonyai, Zsófia
AU - Csonka, Éva Bálintné
AU - Fail, József
AU - Hanks, Lawrence M.
AU - Zou, Yunfan
AU - Millar, Jocelyn G.
AU - Tóth, Miklós
AU - Larsson, Mattias C.
N1 - Funding information The project was supported by research grants from the Swedish Environmental Protection Agency (to MCL, grant number: NV-03135-14); the research council Formas (to MCL, grant number: 2016-01372); the Swedish Forest Society Foundation (to MCL, grants:1011-84 / 150-7 HJHIL and 2016-029); Ekfrämjandet: Erik Stenström Foundation (to MCL, grant: 2015-5); WWF Sweden (grant: Insight: SWE 0163; Local: 500 131); the Royal Swedish Academy of Sciences (to MAM, grant: BS2015-0065); the Higher Education Institutional Excellence Program of the Hungarian Ministry of Human Capacities within the framework of plant breeding and plant protection research (1783-3/2018/FEKUTSTRAT) at Szent István University (to JF and ZL); the János Bolyai Research Scholarship of the Hungarian Academy of Sciences as well as the UNKP Bolyai+ fellowship of the Hungarian Ministry of Human Capacities (to JF); Tranemåla foundation: Erik and Ebba Larsson’s and Thure Rignell’s foundation (to IBW); and by the United States Department of Agriculture, Animal and Plant Health Inspection Service (grants 14-, 15-, 16-, and 17-8130-1422-CA to JGM and LMH).
Open access funding was provided by MTA Centre for Agricultural Research (MTA ATK). We are grateful to Viktor Farkas for the permission and support to carry out experiments in the forests at Piliserdő Zrt., near Pilisszentkereszt. We thank Edit Orgován for assistance during the laboratory experiments and for her analysis of pheromone ratios. Jonas Helgesson at Helgesson Trädtjänst AB, Stefan Ekroth, Gunnar Isacsson, and forest companies Stora Enso and Södra Skogsägarna made significant contributions to the supply of oak wood substrates. Further, we wish to thank Marcus Vestlund, Sunniva Farbu, Adam Nunn, and Shilpi Kundu for assistance in the field with collection of wood substrates and trap maintenance. We thank József Muskovits for taxonomic assistance. We are also grateful to Göran Birgersson for GC-MS support at the Alnarp Campus, and the Swedish forest company Sveaskog (Per Petersson and Jan Dahl) for permission to perform field work within Ecopark Hornsö.
Acknowledgements Open access funding was provided by MTA Centre for Agricultural Research (MTA ATK). We are grateful to Viktor Farkas for the permission and support to carry out experiments in the forests at Piliserdő Zrt., near Pilisszentkereszt. We thank Edit Orgován for assistance during the laboratory experiments and for her analysis of pheromone ratios. Jonas Helgesson at Helgesson Trädtjänst AB, Stefan Ekroth, Gunnar Isacsson, and forest companies Stora Enso and Södra Skogsägarna made significant contributions to the supply of oak wood substrates. Further, we wish to thank Marcus Vestlund, Sunniva Farbu, Adam Nunn, and Shilpi Kundu for assistance in the field with collection of wood substrates and trap maintenance. We thank József Muskovits for taxonomic assistance. We are also grateful to Göran Birgersson for GC-MS support at the Alnarp Campus, and the Swedish forest company Sveaskog (Per Petersson and Jan Dahl) for permission to perform field work within Ecopark Hornsö.
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Plagionotus arcuatus ssp. arcuatus (L.) is a common saproxylic cerambycid beetle in most parts of Europe, and is designated as an occasional pest of oak wood that is stored outside during the summer months. In an effort to identify attractants that can be used for monitoring this species, we collected headspace samples from adult beetles and conducted field bioassays with the resulting compounds as potential aggregation-sex pheromone components for this species. Three compounds, (R)-3-hydroxyhexan-2-one, (R)-3-hydroxyoctan-2-one, and (R)-3-hydroxydecan-2-one, were consistently present in relatively large quantities in headspace extracts from male P. arcuatus populations in both Hungary and Sweden, regardless of the adsorbent media (activated charcoal or Porapak™ Q) used to sample beetle odors, or the type of solvent (hexane, diethyl ether, or dichloromethane) used to elute trapped volatiles from the collectors. None of the hydroxyketone and related compounds were detected in corresponding extracts from females. In field bioassays in both countries, the blend of the C 6 and C 10 compounds, and the ternary blend both attracted significantly more beetles than the control, while other combinations or single compounds were not significantly attractive. Males and females showed similar patterns of responses to treatments. Our results demonstrate that (R)-3-hydroxyhexan-2-one and (R)-3-hydroxydecan-2-one constitute a male-produced aggregation-sex pheromone of P. arcuatus, whereas the role of (R)-3-hydroxyoctan-2-one remains unclear. Lures with the pheromone could be developed for monitoring of P. arcuatus populations as an indicator of fresh oak wood resources.
AB - Plagionotus arcuatus ssp. arcuatus (L.) is a common saproxylic cerambycid beetle in most parts of Europe, and is designated as an occasional pest of oak wood that is stored outside during the summer months. In an effort to identify attractants that can be used for monitoring this species, we collected headspace samples from adult beetles and conducted field bioassays with the resulting compounds as potential aggregation-sex pheromone components for this species. Three compounds, (R)-3-hydroxyhexan-2-one, (R)-3-hydroxyoctan-2-one, and (R)-3-hydroxydecan-2-one, were consistently present in relatively large quantities in headspace extracts from male P. arcuatus populations in both Hungary and Sweden, regardless of the adsorbent media (activated charcoal or Porapak™ Q) used to sample beetle odors, or the type of solvent (hexane, diethyl ether, or dichloromethane) used to elute trapped volatiles from the collectors. None of the hydroxyketone and related compounds were detected in corresponding extracts from females. In field bioassays in both countries, the blend of the C 6 and C 10 compounds, and the ternary blend both attracted significantly more beetles than the control, while other combinations or single compounds were not significantly attractive. Males and females showed similar patterns of responses to treatments. Our results demonstrate that (R)-3-hydroxyhexan-2-one and (R)-3-hydroxydecan-2-one constitute a male-produced aggregation-sex pheromone of P. arcuatus, whereas the role of (R)-3-hydroxyoctan-2-one remains unclear. Lures with the pheromone could be developed for monitoring of P. arcuatus populations as an indicator of fresh oak wood resources.
KW - (R)-3-hydroxydecan-2-one
KW - (R)-3-hydroxyhexan-2-one
KW - (R)-3-hydroxyoctan-2-one
KW - Clytini
KW - Headspace sampling
KW - Isomerization
UR - http://www.scopus.com/inward/record.url?scp=85064952091&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85064952091&partnerID=8YFLogxK
U2 - 10.1007/s00114-019-1612-2
DO - 10.1007/s00114-019-1612-2
M3 - Article
C2 - 31028459
AN - SCOPUS:85064952091
SN - 0028-1042
VL - 106
JO - Science of Nature
JF - Science of Nature
IS - 5-6
M1 - 18
ER -