Identification of sucrose synthase as an actin-binding protein

Heike Winter, Joan L. Huber, Steven C. Huber

Research output: Contribution to journalArticlepeer-review


Several lines of evidence indicate that sucrose synthase (SuSy) binds both G- and F-actin: (i) presence of SuSy in the Triton X-100-insoluble fraction of microsomal membranes (i.e. crude cytoskeleton fraction); (ii) co- immunoprecipitation of actin with anti-SuSy monoclonal antibodies; (iii) association of SuSy with in situ phalloidin-stabilized F-actin filaments; and (iv) direct binding to F-actin, polymerized in vitro. Aldolase, well known to interact with F-actin, interfered with binding of SuSy, suggesting that a common or overlapping binding site may be involved. We postulate that some of the soluble SuSy in the cytosol may be associated with the actin cytoskeleton in vivo.

Original languageEnglish (US)
Pages (from-to)205-208
Number of pages4
JournalFEBS Letters
Issue number3
StatePublished - Jul 3 1998
Externally publishedYes


  • Actin cytoskeleton
  • Actin-binding protein
  • Aldolase
  • Phalloidin
  • Sucrose synthase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


Dive into the research topics of 'Identification of sucrose synthase as an actin-binding protein'. Together they form a unique fingerprint.

Cite this