Identification of RAPD markers associated with the presence of B chromosomes in maize

C. Gourmet, A. Lane Rayburn

Research output: Contribution to journalArticle

Abstract

DNA isolated from Black Mexican sweet maize populations segregating for the presence of B chromosomes was used for PCR amplifications of 320 RAPD primers. Seventeen primers produced detectable polymorphisms between populations known to carry B chromosomes and populations devoid of B chromosomes. To ensure that the primers which generated a polymorphic band were indeed identifying B chromosomes, five Arizona Indian corn populations, three tetraploid corn populations and two High Loss corn lines differing in their B chromosome composition were analysed. Four primers were selected to screen these maize populations. Root tip counts were performed on the lines to confirm the association of the RAPD markers to the presence of B chromosomes.

Original languageEnglish (US)
Pages (from-to)240-244
Number of pages5
JournalHeredity
Volume77
Issue number3
DOIs
StatePublished - Sep 1996

Keywords

  • B chromosomes
  • RAPD markers
  • Zea mays
  • polymorphism

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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