Identification of QTLs associated with agronomic performance under nitrogen-deficient conditions using chromosome segment substitution lines of a wild rice relative, Oryza rufipogon

Satoshi Ogawa, Milton Orlando Valencia, Mathias Lorieux, Juan David Arbelaez, Susan McCouch, Manabu Ishitani, Michael Gomez Selvaraj

Research output: Contribution to journalArticle

Abstract

Improved root system architecture can enhance agronomic performance by increasing water and nitrogen (N) acquisition efficiency. However, little is known about interaction between root system architecture and agronomic performance under field environments. To gain a better understanding about the genetic basis of these relationships, we evaluated a set of chromosome segment substitution lines (CSSLs) derived from crosses between a tropical japonica rice cultivar ‘Curinga’ and a wild species Oryza rufipogon accession IRGC105491. Root system architectural traits were investigated using the CSSLs at 40 days old seedlings using the root basket method under hydroponic conditions, and agronomic performances were also tested under field conditions with different N treatments. Agronomic performances were computed as the ratio of a trait value under low to high N treatments, including grain yield and biomass yield as nitrogen-deficiency tolerance (NDT) traits. Root architecture and NDT trait QTLs were mapped using 238 SNP marker loci. A total of 13 QTLs for root system architectural, NDT and morpho-physiological traits were identified on chromosomes 1, 3, 4, 5, 7, 8, 9, 10 and 12. Interestingly, a QTL for deeper root number was identified the region of SNP markers between id1012330 and id1021697 on chromosome 1 under hydroponic conditions overlapped with a QTL for NDT trait of relative grain yield (qRGY1). These results suggest that deeper root trait is helpful to maintain grain yield under nitrogen-deficient conditions. The QTL associated root architecture could potentially be used in future rice-breeding efforts to increase agronomic performance under nitrogen-deficient conditions.

Original languageEnglish (US)
Article number103
JournalActa Physiologiae Plantarum
Volume38
Issue number4
DOIs
StatePublished - Apr 1 2016
Externally publishedYes

Fingerprint

Oryza rufipogon
wild rice
substitution lines
agronomic traits
quantitative trait loci
Nitrogen
Chromosomes
chromosomes
nitrogen
root systems
Hydroponics
grain yield
Chromosomes, Human, Pair 1
hydroponics
Single Nucleotide Polymorphism
rice
Chromosomes, Human, Pair 3
Oryza
Seedlings
Biomass

Keywords

  • Deeper root
  • Nitrogen-deficiency tolerance
  • Quantitative trait locus
  • Root system architecture
  • Underground revolution

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Identification of QTLs associated with agronomic performance under nitrogen-deficient conditions using chromosome segment substitution lines of a wild rice relative, Oryza rufipogon. / Ogawa, Satoshi; Valencia, Milton Orlando; Lorieux, Mathias; Arbelaez, Juan David; McCouch, Susan; Ishitani, Manabu; Selvaraj, Michael Gomez.

In: Acta Physiologiae Plantarum, Vol. 38, No. 4, 103, 01.04.2016.

Research output: Contribution to journalArticle

Ogawa, Satoshi ; Valencia, Milton Orlando ; Lorieux, Mathias ; Arbelaez, Juan David ; McCouch, Susan ; Ishitani, Manabu ; Selvaraj, Michael Gomez. / Identification of QTLs associated with agronomic performance under nitrogen-deficient conditions using chromosome segment substitution lines of a wild rice relative, Oryza rufipogon. In: Acta Physiologiae Plantarum. 2016 ; Vol. 38, No. 4.
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