Identification of pathogenic bacteria in mixed cultures by FTIR spectroscopy

Research output: Contribution to journalArticlepeer-review

Abstract

Routine identification of pathogenic microorganisms predominantly based on nutritional and biochemical tests is a time-consuming process. In this study, a method based on FTIR spectroscopy was developed to detect and identify the presence of five possible pathogenic bacteria in ten different microorganism mixtures with each cocktail containing up to three different species at a concentration of 10 9 CFU/mL. A mathematical approach based on factoring out the common features in the spectral fingerprints was developed and implemented in conjunction with discriminant analysis. FTIR spectra of the mixtures were directly obtained and analyzed using canonical variate analysis based on the discriminant model for the presence of particular species in the pathogenic bacteria mixtures. In nine out often mixtures, the predictions were 100% accurate; in one mixture, only one false negative was reported, with no false positives. Results suggest that FTIR spectroscopy combined with a suitable analytical procedure has excellent potential as a fast, powerful, and reliable alternative for identifying a specific foodborne pathogen in a complex and/or competing system.

Original languageEnglish (US)
Pages (from-to)1623-1632
Number of pages10
JournalTransactions of the ASABE
Volume49
Issue number5
StatePublished - Sep 1 2006
Externally publishedYes

Keywords

  • Bacteria identification
  • FT-IR spectroscopy

ASJC Scopus subject areas

  • Forestry
  • Food Science
  • Biomedical Engineering
  • Agronomy and Crop Science
  • Soil Science

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