Identification of novel double-stranded RNA mycoviruses of Fusarium virguliforme and evidence of their effects on virulence

Rita A. Marvelli; Houston A. Hobbs; Shuxian Li; Nancy K. McCoppin; Leslie L. Domier; Glen L. Hartman; Darin Eastburn

doi:10.1007/s00705-013-1760-1

Identification of novel double-stranded RNA mycoviruses of Fusarium virguliforme and evidence of their effects on virulence

Rita A. Marvelli, Houston A. Hobbs, Shuxian Li, Nancy K. McCoppin, Leslie L. Domier, Glen L. Hartman, Darin Eastburn

Crop Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Virulence and double-stranded RNA (dsRNA) profiles of 44 isolates of Fusarium virguliforme were compared. When grouped according to dsRNA profiles, isolates with large dsRNAs were significantly (P≤0.05) less virulent than isolates without dsRNAs. High-throughput sequence analysis of total RNA prepared from cultures with large dsRNAs identified two novel RNA viruses with genome sequences of approximately 9.3 kbp, which were named Fusarium virguliforme dsRNA mycovirus 1 and Fusarium virguliforme dsRNA mycovirus 2. The new viruses were most closely related to a group of unclassified viruses that included viruses of F. graminearum and Phlebiopsis gigantea and are related to members of the family Totiviridae.

Original language	English (US)
Pages (from-to)	349-352
Number of pages	4
Journal	Archives of Virology
Volume	159
Issue number	2
DOIs	https://doi.org/10.1007/s00705-013-1760-1
State	Published - Feb 2014

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Virology

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10.1007/s00705-013-1760-1

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title = "Identification of novel double-stranded RNA mycoviruses of Fusarium virguliforme and evidence of their effects on virulence",

abstract = "Virulence and double-stranded RNA (dsRNA) profiles of 44 isolates of Fusarium virguliforme were compared. When grouped according to dsRNA profiles, isolates with large dsRNAs were significantly (P≤0.05) less virulent than isolates without dsRNAs. High-throughput sequence analysis of total RNA prepared from cultures with large dsRNAs identified two novel RNA viruses with genome sequences of approximately 9.3 kbp, which were named Fusarium virguliforme dsRNA mycovirus 1 and Fusarium virguliforme dsRNA mycovirus 2. The new viruses were most closely related to a group of unclassified viruses that included viruses of F. graminearum and Phlebiopsis gigantea and are related to members of the family Totiviridae.",

author = "Marvelli, {Rita A.} and Hobbs, {Houston A.} and Shuxian Li and McCoppin, {Nancy K.} and Domier, {Leslie L.} and Hartman, {Glen L.} and Darin Eastburn",

note = "Funding Information: We thank A. M. Dorrance, W. L. Pedersen, and X. B. Yang for providing F. virguliforme isolates, J. Chee-Sanford and K. N. Lambert for use of gel imaging systems, W. Chen, C. J. D{\textquoteright}Arcy, and K. N. Paige for their research advice, and W. A. Maffit for assistance in total RNA purifications. Funding for this project was provided, in part, from the USDA NC-125 project, The Illinois Soybean Association, and from the University of Illinois at Urbana Champaign Campus Research Board.",

year = "2014",

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doi = "10.1007/s00705-013-1760-1",

language = "English (US)",

volume = "159",

pages = "349--352",

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T1 - Identification of novel double-stranded RNA mycoviruses of Fusarium virguliforme and evidence of their effects on virulence

AU - Marvelli, Rita A.

AU - Hobbs, Houston A.

AU - Li, Shuxian

AU - McCoppin, Nancy K.

AU - Domier, Leslie L.

AU - Hartman, Glen L.

AU - Eastburn, Darin

N1 - Funding Information: We thank A. M. Dorrance, W. L. Pedersen, and X. B. Yang for providing F. virguliforme isolates, J. Chee-Sanford and K. N. Lambert for use of gel imaging systems, W. Chen, C. J. D’Arcy, and K. N. Paige for their research advice, and W. A. Maffit for assistance in total RNA purifications. Funding for this project was provided, in part, from the USDA NC-125 project, The Illinois Soybean Association, and from the University of Illinois at Urbana Champaign Campus Research Board.

PY - 2014/2

Y1 - 2014/2

N2 - Virulence and double-stranded RNA (dsRNA) profiles of 44 isolates of Fusarium virguliforme were compared. When grouped according to dsRNA profiles, isolates with large dsRNAs were significantly (P≤0.05) less virulent than isolates without dsRNAs. High-throughput sequence analysis of total RNA prepared from cultures with large dsRNAs identified two novel RNA viruses with genome sequences of approximately 9.3 kbp, which were named Fusarium virguliforme dsRNA mycovirus 1 and Fusarium virguliforme dsRNA mycovirus 2. The new viruses were most closely related to a group of unclassified viruses that included viruses of F. graminearum and Phlebiopsis gigantea and are related to members of the family Totiviridae.

AB - Virulence and double-stranded RNA (dsRNA) profiles of 44 isolates of Fusarium virguliforme were compared. When grouped according to dsRNA profiles, isolates with large dsRNAs were significantly (P≤0.05) less virulent than isolates without dsRNAs. High-throughput sequence analysis of total RNA prepared from cultures with large dsRNAs identified two novel RNA viruses with genome sequences of approximately 9.3 kbp, which were named Fusarium virguliforme dsRNA mycovirus 1 and Fusarium virguliforme dsRNA mycovirus 2. The new viruses were most closely related to a group of unclassified viruses that included viruses of F. graminearum and Phlebiopsis gigantea and are related to members of the family Totiviridae.

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Identification of novel double-stranded RNA mycoviruses of Fusarium virguliforme and evidence of their effects on virulence

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