Identification of Nedotepa curta Dmitriev as a potential vector of the Côte d'Ivoire lethal yellowing phytoplasma in coconut palms sole or in mixed infection with a ‘Candidatus Phytoplasma asteris’-related strain

Koffi Eric Kwadjo, N. ’Djiha Isabelle Beugré, Christopher H Dietrich, Adaba Tano Thierry Kodjo, Hortense Atta Diallo, Ndede Yankey, Sylvester Dery, Michael Wilson, Jean Louis Konan Konan, Nicoletta Contaldo, Samanta Paltrinieri, Assunta Bertaccini, Yaima Arocha Rosete

Research output: Contribution to journalArticle

Abstract

Over 360 Hemiptera specimens were collected using sweep nets and hand-made aspirators from coconut palm fronds in six villages of Grand-Lahou. Eight families were identified including Aphrophoridae, Achilidae, Derbidae, Flatidae, Membracidae, Pentatomidae, Tropiduchidae, and Cicadellidae, the latter being the most abundant throughout the surveyed villages. PCR assays with primers targeting the 16S rRNA and the secA translocation protein genes yielded PCR amplicons from 216 out of 296 (73%) of the tested specimens of a newly identified cicadellid leafhopper, Nedotepa curta Dmitriev. PCR amplicons were purified, cloned and sequenced. The 16S rDNA and secA sequences from N. curta showed a 99% of sequence identity with those of the Côte d'Ivoire lethal yellowing (CILY) phytoplasma, member of subgroup 16SrXXII-B ‘Candidatus Phytoplasma palmicola’-related strain, previously identified in coconut-growing villages of Grand-Lahou. This suggested N. curta as a potential vector for the CILY phytoplasma. Four symptomatic coconut palms (7.4%) were found infected by a phytoplasma of group 16SrI, in mixed infection with the CILY phytoplasma (16SrXXII-B) in two palms, and alone in the other two palms, where the CILY phytoplasma was not detected. The 16SrI phytoplasma was also found in two N. curta specimens, and in the weeds Dalbergia saxatilis and Baphia nitida. Results indicating that mixed infection of both 16SrXXII-B and 16SrI phytoplasmas occurs in coconut palms affected by CILY in Grand-Lahou, and may impact disease management and control.

Original languageEnglish (US)
Pages (from-to)48-56
Number of pages9
JournalCrop Protection
Volume110
DOIs
StatePublished - Aug 2018

Fingerprint

lethal yellowing
Cocos nucifera
Phytoplasma
mixed infection
16SrI (Aster yellows group)
villages
Cicadellidae
Derbidae
Baphia
disease control
Aphrophoridae
Flatidae
Tropiduchidae
aspirators
Membracidae
vector competence
protein transport
Pentatomidae
fronds
coconuts

Keywords

  • 16SrXXII
  • Coconut lethal yellowing
  • Côte d'Ivoire
  • Nedotepa curta
  • Phytoplasma
  • Potential vector

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Identification of Nedotepa curta Dmitriev as a potential vector of the Côte d'Ivoire lethal yellowing phytoplasma in coconut palms sole or in mixed infection with a ‘Candidatus Phytoplasma asteris’-related strain. / Kwadjo, Koffi Eric; Beugré, N. ’Djiha Isabelle; Dietrich, Christopher H; Thierry Kodjo, Adaba Tano; Atta Diallo, Hortense; Yankey, Ndede; Dery, Sylvester; Wilson, Michael; Konan Konan, Jean Louis; Contaldo, Nicoletta; Paltrinieri, Samanta; Bertaccini, Assunta; Arocha Rosete, Yaima.

In: Crop Protection, Vol. 110, 08.2018, p. 48-56.

Research output: Contribution to journalArticle

Kwadjo, KE, Beugré, NDI, Dietrich, CH, Thierry Kodjo, AT, Atta Diallo, H, Yankey, N, Dery, S, Wilson, M, Konan Konan, JL, Contaldo, N, Paltrinieri, S, Bertaccini, A & Arocha Rosete, Y 2018, 'Identification of Nedotepa curta Dmitriev as a potential vector of the Côte d'Ivoire lethal yellowing phytoplasma in coconut palms sole or in mixed infection with a ‘Candidatus Phytoplasma asteris’-related strain', Crop Protection, vol. 110, pp. 48-56. https://doi.org/10.1016/j.cropro.2017.12.015
Kwadjo, Koffi Eric ; Beugré, N. ’Djiha Isabelle ; Dietrich, Christopher H ; Thierry Kodjo, Adaba Tano ; Atta Diallo, Hortense ; Yankey, Ndede ; Dery, Sylvester ; Wilson, Michael ; Konan Konan, Jean Louis ; Contaldo, Nicoletta ; Paltrinieri, Samanta ; Bertaccini, Assunta ; Arocha Rosete, Yaima. / Identification of Nedotepa curta Dmitriev as a potential vector of the Côte d'Ivoire lethal yellowing phytoplasma in coconut palms sole or in mixed infection with a ‘Candidatus Phytoplasma asteris’-related strain. In: Crop Protection. 2018 ; Vol. 110. pp. 48-56.
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abstract = "Over 360 Hemiptera specimens were collected using sweep nets and hand-made aspirators from coconut palm fronds in six villages of Grand-Lahou. Eight families were identified including Aphrophoridae, Achilidae, Derbidae, Flatidae, Membracidae, Pentatomidae, Tropiduchidae, and Cicadellidae, the latter being the most abundant throughout the surveyed villages. PCR assays with primers targeting the 16S rRNA and the secA translocation protein genes yielded PCR amplicons from 216 out of 296 (73{\%}) of the tested specimens of a newly identified cicadellid leafhopper, Nedotepa curta Dmitriev. PCR amplicons were purified, cloned and sequenced. The 16S rDNA and secA sequences from N. curta showed a 99{\%} of sequence identity with those of the C{\^o}te d'Ivoire lethal yellowing (CILY) phytoplasma, member of subgroup 16SrXXII-B ‘Candidatus Phytoplasma palmicola’-related strain, previously identified in coconut-growing villages of Grand-Lahou. This suggested N. curta as a potential vector for the CILY phytoplasma. Four symptomatic coconut palms (7.4{\%}) were found infected by a phytoplasma of group 16SrI, in mixed infection with the CILY phytoplasma (16SrXXII-B) in two palms, and alone in the other two palms, where the CILY phytoplasma was not detected. The 16SrI phytoplasma was also found in two N. curta specimens, and in the weeds Dalbergia saxatilis and Baphia nitida. Results indicating that mixed infection of both 16SrXXII-B and 16SrI phytoplasmas occurs in coconut palms affected by CILY in Grand-Lahou, and may impact disease management and control.",
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AU - Kwadjo, Koffi Eric

AU - Beugré, N. ’Djiha Isabelle

AU - Dietrich, Christopher H

AU - Thierry Kodjo, Adaba Tano

AU - Atta Diallo, Hortense

AU - Yankey, Ndede

AU - Dery, Sylvester

AU - Wilson, Michael

AU - Konan Konan, Jean Louis

AU - Contaldo, Nicoletta

AU - Paltrinieri, Samanta

AU - Bertaccini, Assunta

AU - Arocha Rosete, Yaima

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AB - Over 360 Hemiptera specimens were collected using sweep nets and hand-made aspirators from coconut palm fronds in six villages of Grand-Lahou. Eight families were identified including Aphrophoridae, Achilidae, Derbidae, Flatidae, Membracidae, Pentatomidae, Tropiduchidae, and Cicadellidae, the latter being the most abundant throughout the surveyed villages. PCR assays with primers targeting the 16S rRNA and the secA translocation protein genes yielded PCR amplicons from 216 out of 296 (73%) of the tested specimens of a newly identified cicadellid leafhopper, Nedotepa curta Dmitriev. PCR amplicons were purified, cloned and sequenced. The 16S rDNA and secA sequences from N. curta showed a 99% of sequence identity with those of the Côte d'Ivoire lethal yellowing (CILY) phytoplasma, member of subgroup 16SrXXII-B ‘Candidatus Phytoplasma palmicola’-related strain, previously identified in coconut-growing villages of Grand-Lahou. This suggested N. curta as a potential vector for the CILY phytoplasma. Four symptomatic coconut palms (7.4%) were found infected by a phytoplasma of group 16SrI, in mixed infection with the CILY phytoplasma (16SrXXII-B) in two palms, and alone in the other two palms, where the CILY phytoplasma was not detected. The 16SrI phytoplasma was also found in two N. curta specimens, and in the weeds Dalbergia saxatilis and Baphia nitida. Results indicating that mixed infection of both 16SrXXII-B and 16SrI phytoplasmas occurs in coconut palms affected by CILY in Grand-Lahou, and may impact disease management and control.

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