Identification of key regulatory pathways of myeloid differentiation using an mESC-based karyotypically normal cell model

Dong Li, Hong Yang, Hong Nan, Peng Liu, Sulei Pang, Qian Zhao, Rotem Karni, Mark P. Kamps, Yuanfu Xu, Jiaxi Zhou, Therese Wiedmer, Peter J. Sims, Fei Wang

Research output: Contribution to journalArticle

Abstract

Understanding the process of myeloid differentiation offers important insights into both normal and abnormal developmental processes but is limited by the dearth of experimental models. Here we show that myeloid progenitors can be derived from embryonic stem cells, immortalized, and applied to the study of the mechanisms underlying myeloid differentiation. The embryonic stem cell-derived myeloid progenitors, when immortalized with estrogen-regulated Hoxb8 protein, demonstrate normal karyotyping, are genetically tractable, and can be differentiated into functional neutrophils. Using this model, we identified mammalian target of rapamycin complex 1 as a critical regulator of myeloid differentiation.Together, our studies led to a convenient, karyotypically normal, and genetically manipulatable cellular system, which can be used to shed new light on the mechanisms for myeloid differentiation.

Original languageEnglish (US)
Pages (from-to)4712-4719
Number of pages8
JournalBlood
Volume120
Issue number24
DOIs
StatePublished - Dec 6 2012

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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  • Cite this

    Li, D., Yang, H., Nan, H., Liu, P., Pang, S., Zhao, Q., Karni, R., Kamps, M. P., Xu, Y., Zhou, J., Wiedmer, T., Sims, P. J., & Wang, F. (2012). Identification of key regulatory pathways of myeloid differentiation using an mESC-based karyotypically normal cell model. Blood, 120(24), 4712-4719. https://doi.org/10.1182/blood-2012-03-414979