TY - JOUR
T1 - Identification of key immune mediators regulating T helper 1 responses in swine
AU - Dawson, Harry D.
AU - Royaee, Atabak R.
AU - Nishi, Sandra
AU - Kuhar, Daniel
AU - Schnitzlein, William M.
AU - Zuckermann, Federico
AU - Urban, Joseph
AU - Lunney, Joan K.
N1 - Funding Information:
The authors thank Dr. Jim Higgins, Ms. Christina Hahn and Mr. Sebastian Botero, EMSL, ANRI, BARC, for helpful assistance with sequencing the swine genes and Dr. J.P. Dubey for supplying oocysts for the pig infections. This work was supported by USDA ARS CRIS funds and by a grant from the Biotechnology Research and Development Corporation to JL and FZ.
PY - 2004/7
Y1 - 2004/7
N2 - This publication describes the cloning of full or partial length sequences for pig TBX21 (T-bet), MYD88, ICSBP1, CD8A (CD8α), CD8B (CD8β), and CD28 cDNAs. Real-time PCR assays have been developed for the relative quantitation of these products as well as previously characterized transcripts that encode exon A-containing CD45, HLX1, IRF1, STAT1 and RPL32. When used for examining temporal immune gene expression in the liver of Toxoplasma gondii infected pigs, the positive regulators of Th1 responses, IRF1, MYD88, and STAT1, were found to be expressed prior to the simultaneous upregulation of interferon gamma (IFNG), HLX1 and TBX21 gene expression. In contrast, in the mesenteric lymph node (MLN), only expression of IRF1 and IFNG was significantly upregulated. Based on their demonstrated utility in establishing an immune response pathway, these PCR assays should be valuable additions to our swine immune toolkit.
AB - This publication describes the cloning of full or partial length sequences for pig TBX21 (T-bet), MYD88, ICSBP1, CD8A (CD8α), CD8B (CD8β), and CD28 cDNAs. Real-time PCR assays have been developed for the relative quantitation of these products as well as previously characterized transcripts that encode exon A-containing CD45, HLX1, IRF1, STAT1 and RPL32. When used for examining temporal immune gene expression in the liver of Toxoplasma gondii infected pigs, the positive regulators of Th1 responses, IRF1, MYD88, and STAT1, were found to be expressed prior to the simultaneous upregulation of interferon gamma (IFNG), HLX1 and TBX21 gene expression. In contrast, in the mesenteric lymph node (MLN), only expression of IRF1 and IFNG was significantly upregulated. Based on their demonstrated utility in establishing an immune response pathway, these PCR assays should be valuable additions to our swine immune toolkit.
KW - Cytokine gene regulation
KW - Protozoan parasite
KW - Swine
KW - T helper 1 immunity
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U2 - 10.1016/j.vetimm.2004.03.006
DO - 10.1016/j.vetimm.2004.03.006
M3 - Article
C2 - 15183000
AN - SCOPUS:2942566292
SN - 0165-2427
VL - 100
SP - 105
EP - 111
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
IS - 1-2
ER -