Identification of immunodominant T-cell epitopes present in glycoprotein 5 of the North American genotype of porcine reproductive and respiratory syndrome virus

Kapil Vashisht, Tony L. Goldberg, Robert J. Husmann, William Schnitzlein, Federico A. Zuckermann

Research output: Contribution to journalArticlepeer-review

Abstract

Ninty-six pentadecapeptides spanning glycoprotein 5 (GP5) of porcine reproductive and respiratory virus (PRRSV) were screened for their ability to elicit a recall interferon-γ response from peripheral blood mononuclear cells isolated from 22 pigs infected with up to two genetically divergent PRRSV strains. Two distinct regions (amino acid residues 117-131, LAALICFVIRLAKNC, and 149-163, KGRLYRWRSPVII/VEK) appeared to contain immunodominant T-cell epitopes based on their ability to stimulate above average numbers of interferon-γ secreting cells as compared to other GP5 peptides. A survey of PRRSV isolates indicated that these two sites are relatively conserved with at most a two amino acid variation and thus should be considered for incorporation into a multi-valent vaccine against PRRS.

Original languageEnglish (US)
Pages (from-to)4747-4753
Number of pages7
JournalVaccine
Volume26
Issue number36
DOIs
StatePublished - Aug 26 2008

Keywords

  • Glycoprotein-5
  • Porcine reproductive and respiratory syndrome virus
  • T-cell epitopes

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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