Identification of GtgE, a novel virulence factor encoded on the Gifsy-2 bacteriophage of Salmonella enterica serovar typhimurium

Theresa D. Ho, Nara Figueroa-Bossi, Minhua Wang, Sergio Uzzau, Lionello Bossi, James M. Slauch

Research output: Contribution to journalArticle

Abstract

The Gifsy-2 temperate bacteriophage of Salmonella enterica serovar Typhimurium contributes significantly to the pathogenicity of strains that carry it as a prophage. Previous studies have shown that Gifsy-2 encodes SodCI, a periplasmic Cu/Zn superoxide dismutase, and at least one additional virulence factor. Gifsy-2 encodes a Salmonella pathogenicity island 2 type III secreted effector protein. Sequence analysis of the Gifsy-2 genome also identifies several open reading frames with homology to those of known virulence genes. However, we found that null mutations in these genes did not individually have a significant effect on the ability of S. enterica serovar Typhimurium to establish a systemic infection in mice. Using deletion analysis, we have identified a gene, gtgE, which is necessary for the full virulence of S. enterica serovar Typhimurium Gifsy-2 lysogens. Together, GtgE and SodCI account for the contribution of Gifsy-2 to S. enterica serovar Typhimurium virulence in the murine model.

Original languageEnglish (US)
Pages (from-to)5234-5239
Number of pages6
JournalJournal of bacteriology
Volume184
Issue number19
DOIs
StatePublished - Oct 1 2002

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Salmonella enterica
Virulence Factors
Bacteriophages
Virulence
Genes
Prophages
Genomic Islands
Salmonella
Open Reading Frames
Sequence Analysis
Genome
Mutation
Serogroup
Infection
Proteins

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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Identification of GtgE, a novel virulence factor encoded on the Gifsy-2 bacteriophage of Salmonella enterica serovar typhimurium. / Ho, Theresa D.; Figueroa-Bossi, Nara; Wang, Minhua; Uzzau, Sergio; Bossi, Lionello; Slauch, James M.

In: Journal of bacteriology, Vol. 184, No. 19, 01.10.2002, p. 5234-5239.

Research output: Contribution to journalArticle

Ho, Theresa D. ; Figueroa-Bossi, Nara ; Wang, Minhua ; Uzzau, Sergio ; Bossi, Lionello ; Slauch, James M. / Identification of GtgE, a novel virulence factor encoded on the Gifsy-2 bacteriophage of Salmonella enterica serovar typhimurium. In: Journal of bacteriology. 2002 ; Vol. 184, No. 19. pp. 5234-5239.
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