Identification of GtgE, a novel virulence factor encoded on the Gifsy-2 bacteriophage of Salmonella enterica serovar typhimurium

Theresa D. Ho, Nara Figueroa-Bossi, Minhua Wang, Sergio Uzzau, Lionello Bossi, James M. Slauch

Research output: Contribution to journalArticle

Abstract

The Gifsy-2 temperate bacteriophage of Salmonella enterica serovar Typhimurium contributes significantly to the pathogenicity of strains that carry it as a prophage. Previous studies have shown that Gifsy-2 encodes SodCI, a periplasmic Cu/Zn superoxide dismutase, and at least one additional virulence factor. Gifsy-2 encodes a Salmonella pathogenicity island 2 type III secreted effector protein. Sequence analysis of the Gifsy-2 genome also identifies several open reading frames with homology to those of known virulence genes. However, we found that null mutations in these genes did not individually have a significant effect on the ability of S. enterica serovar Typhimurium to establish a systemic infection in mice. Using deletion analysis, we have identified a gene, gtgE, which is necessary for the full virulence of S. enterica serovar Typhimurium Gifsy-2 lysogens. Together, GtgE and SodCI account for the contribution of Gifsy-2 to S. enterica serovar Typhimurium virulence in the murine model.

Original languageEnglish (US)
Pages (from-to)5234-5239
Number of pages6
JournalJournal of bacteriology
Volume184
Issue number19
DOIs
StatePublished - Oct 1 2002

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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