Identification of estradiol/ERα-regulated genes in the mouse pituitary

Hyun Joon Kim, Mary C. Gieske, Kourtney L. Trudgen, Susan Hudgins-Spivey, Beob Gyun Kim, Andree Krust, Pierre Chambon, Jae Wook Jeong, Eric Blalock, Che Myong Ko

Research output: Contribution to journalArticle

Abstract

Estrogen acts to prime the pituitary prior to the GnRHinduced LH surge by undiscovered mechanisms. This study aimed to identify the key components that mediate estrogen action in priming the pituitary. RNA extracted from the pituitaries of metestrous (low estrogen) and proestrus (high estrogen) stage mice, as well as from ovariectomized wildtype and estrogen receptor α (ERα) knockout mice treated with 17β-estradiol (E2) or vehicle, was used for gene expression microarray. Microarray data were then aggregated, built into a functional electronic database, and used for further characterization of E2/ERα-regulated genes. These data were used to compile a list of genes representing diverse biological pathways that are regulated by E2 via an ERα-mediated pathway in the pituitary. This approach substantiates ERα regulation of membrane potential regulators and intracellular vesicle transporters, among others, but not the basic components of secretory machinery. Subsequent characterization of six selected genes (Cacna1a, Cacna1g, Cited1, Abep1, Opn3, and Kcne2) confirmed not only ERα dependency for their pituitary expression but also the significance of their expression in regulating GnRH-induced LH secretion. In conclusion, findings from this study suggest that estrogen primes the pituitary via ERα by equipping pituitary cells with critical cellular components that potentiate LH release on subsequent GnRH stimulations.

Original languageEnglish (US)
Pages (from-to)309-321
Number of pages13
JournalJournal of Endocrinology
Volume210
Issue number3
DOIs
StatePublished - Sep 1 2011
Externally publishedYes

Fingerprint

Estradiol Receptors
Estrogen Receptors
Estrogens
Genes
Gonadotropin-Releasing Hormone
Secretory Component
Proestrus
Knockout Mice
Membrane Potentials
Estradiol
Databases
RNA
Gene Expression

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Kim, H. J., Gieske, M. C., Trudgen, K. L., Hudgins-Spivey, S., Kim, B. G., Krust, A., ... Ko, C. M. (2011). Identification of estradiol/ERα-regulated genes in the mouse pituitary. Journal of Endocrinology, 210(3), 309-321. https://doi.org/10.1530/JOE-11-0098

Identification of estradiol/ERα-regulated genes in the mouse pituitary. / Kim, Hyun Joon; Gieske, Mary C.; Trudgen, Kourtney L.; Hudgins-Spivey, Susan; Kim, Beob Gyun; Krust, Andree; Chambon, Pierre; Jeong, Jae Wook; Blalock, Eric; Ko, Che Myong.

In: Journal of Endocrinology, Vol. 210, No. 3, 01.09.2011, p. 309-321.

Research output: Contribution to journalArticle

Kim, HJ, Gieske, MC, Trudgen, KL, Hudgins-Spivey, S, Kim, BG, Krust, A, Chambon, P, Jeong, JW, Blalock, E & Ko, CM 2011, 'Identification of estradiol/ERα-regulated genes in the mouse pituitary', Journal of Endocrinology, vol. 210, no. 3, pp. 309-321. https://doi.org/10.1530/JOE-11-0098
Kim HJ, Gieske MC, Trudgen KL, Hudgins-Spivey S, Kim BG, Krust A et al. Identification of estradiol/ERα-regulated genes in the mouse pituitary. Journal of Endocrinology. 2011 Sep 1;210(3):309-321. https://doi.org/10.1530/JOE-11-0098
Kim, Hyun Joon ; Gieske, Mary C. ; Trudgen, Kourtney L. ; Hudgins-Spivey, Susan ; Kim, Beob Gyun ; Krust, Andree ; Chambon, Pierre ; Jeong, Jae Wook ; Blalock, Eric ; Ko, Che Myong. / Identification of estradiol/ERα-regulated genes in the mouse pituitary. In: Journal of Endocrinology. 2011 ; Vol. 210, No. 3. pp. 309-321.
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