Identification of CT46/HORMAD1, an immunogenic cancer/testis antigen encoding a putative meiosis-related protein

Yao Tseng Chen, Charis A. Venditti, Gregory Theiler, Brian J. Stevenson, Christian Iseli, Ali O. Gure, C. Victor Jongeneel, Lloyd J. Old, Andrew J.G. Simpson

Research output: Contribution to journalArticlepeer-review

Abstract

Transcripts with ESTs derived exclusively or predominantly from testis, and not from other normal tissues, are likely to be products of genes with testis-restricted expression, and are thus potential cancer/testis (CT) antigen genes. A list of 371 genes with such characteristics was compiled by analyzing publicly available EST databases. RT-PCR analysis of normal and tumor tissues was performed to validate an initial selection of 20 of these genes. Several new CT and CT-like genes were identified. One of these, CT46/HORMAD1, is expressed strongly in testis and weakly in placenta; the highest level of expression in other tissues is <1% of testicular expression. The CT46/HORMAD1 gene was expressed in 31% (34/109) of the carcinomas examined, with 11% (12/109) showing expression levels >10% of the testicular level of expression. CT46/HORMAD1 is a single-copy gene on chromosome 1q21.3, encoding a putative protein of 394 aa. Conserved protein domain analysis identified a HORMA domain involved in chromatin binding. The CT46/HORMAD1 protein was found to be homologous to the prototype HORMA domain-containing protein, Hop1, a yeast meiosis-specific protein, as well as to asy1, a meiotic synaptic mutant protein in Arabidopsis thaliana.

Original languageEnglish (US)
Article number9
JournalCancer Immunity
Volume5
StatePublished - Jul 7 2005

Keywords

  • Cancer/testis
  • ESTs
  • HORMAD1
  • Human
  • Tissue distribution
  • Tumor antigens
  • mRNA

ASJC Scopus subject areas

  • Immunology
  • Cancer Research

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