Identification of clustered YY1 binding sites in imprinting control regions

Jeong Do Kim, Angela K. Hinz, Anne Bergmann, Jennifer M. Huang, Ivan Ovcharenko, Lisa Stubbs, Joomyeong Kim

Research output: Contribution to journalArticle

Abstract

Mammalian genomic imprinting is regulated by imprinting control regions (ICRs) that are usually associated with tandem arrays of transcription factor binding sites. In this study, the sequence features derived from a tandem array of YY1 binding sites of Peg3-DMR (differentially methylated region) led us to identify three additional clustered YY1 binding sites, which are also localized within the DMRs of Xist, Tsix, and Nespas. These regions have been shown to play a critical role as ICRs for the regulation of surrounding genes. These ICRs have maintained a tandem array of YY1 binding sites during mammalian evolution. The in vivo binding of YY1 to these regions is allele specific and only to the unmethylated active alleles. Promoter/enhancer assays suggest that a tandem array of YY1 binding sites function as a potential orientation-dependent enhancer. Insulator assays revealed that the enhancer-blocking activity is detected only in the YY1 binding sites of Peg3-DMR but not in the YY1 binding sites of other DMRs. Overall, our identification of three additional clustered YY1 binding sites in imprinted domains suggests a significant role for YY1 in mammalian genomic imprinting.

Original languageEnglish (US)
Pages (from-to)901-911
Number of pages11
JournalGenome Research
Volume16
Issue number7
DOIs
StatePublished - Jul 10 2006
Externally publishedYes

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Binding Sites
Genomic Imprinting
Alleles
Transcription Factors
Genes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Kim, J. D., Hinz, A. K., Bergmann, A., Huang, J. M., Ovcharenko, I., Stubbs, L., & Kim, J. (2006). Identification of clustered YY1 binding sites in imprinting control regions. Genome Research, 16(7), 901-911. https://doi.org/10.1101/gr.5091406

Identification of clustered YY1 binding sites in imprinting control regions. / Kim, Jeong Do; Hinz, Angela K.; Bergmann, Anne; Huang, Jennifer M.; Ovcharenko, Ivan; Stubbs, Lisa; Kim, Joomyeong.

In: Genome Research, Vol. 16, No. 7, 10.07.2006, p. 901-911.

Research output: Contribution to journalArticle

Kim, JD, Hinz, AK, Bergmann, A, Huang, JM, Ovcharenko, I, Stubbs, L & Kim, J 2006, 'Identification of clustered YY1 binding sites in imprinting control regions', Genome Research, vol. 16, no. 7, pp. 901-911. https://doi.org/10.1101/gr.5091406
Kim JD, Hinz AK, Bergmann A, Huang JM, Ovcharenko I, Stubbs L et al. Identification of clustered YY1 binding sites in imprinting control regions. Genome Research. 2006 Jul 10;16(7):901-911. https://doi.org/10.1101/gr.5091406
Kim, Jeong Do ; Hinz, Angela K. ; Bergmann, Anne ; Huang, Jennifer M. ; Ovcharenko, Ivan ; Stubbs, Lisa ; Kim, Joomyeong. / Identification of clustered YY1 binding sites in imprinting control regions. In: Genome Research. 2006 ; Vol. 16, No. 7. pp. 901-911.
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