Identification of a Ferryl Intermediate of Escherichia coli Cytochrome d Terminal Oxidase by Resonance Raman Spectroscopy

Michael A. Kahlow, Thomas M. Loehr, Tamma M. Zuberi, Robert B. Gennis

Research output: Contribution to journalArticle

Abstract

The 680-nm-absorbing “peroxide state” of the Escherichia coli cytochrome d terminal oxidase complex, obtained by addition of excess hydrogen peroxide to the enzyme, is shown to be a ferryl intermediate in the catalytic cycle of the enzyme. This ferryl intermediate is also created by aerobic oxidation of the fully reduced enzyme. Resonance Raman spectra with 647.1-nm excitation show an FeIV=O stretching band at 815 cm-1, a higher frequency than noted in any other ferrylcontaining enzyme to date. The band shows an 16O/18O frequency shift of -46 cm-1, larger than that observed for any porphyrin ferryl species. The FeIV=O formulation was unambiguously established by oxidations of the reduced enzyme with 16O2, 18O2, and 16O18O. Only the use of a mixed-isotope gas permitted discrimination between a ferryl and a peroxo structure. A catalytic cycle for the cytochrome d terminal oxidase complex is proposed, and possible reasons for the high v(Fe=O) frequency are discussed.

Original languageEnglish (US)
Pages (from-to)11485-11489
Number of pages5
JournalBiochemistry
Volume30
Issue number49
DOIs
StatePublished - Dec 1 1991

ASJC Scopus subject areas

  • Biochemistry

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