Abstract
The 680-nm-absorbing “peroxide state” of the Escherichia coli cytochrome d terminal oxidase complex, obtained by addition of excess hydrogen peroxide to the enzyme, is shown to be a ferryl intermediate in the catalytic cycle of the enzyme. This ferryl intermediate is also created by aerobic oxidation of the fully reduced enzyme. Resonance Raman spectra with 647.1-nm excitation show an FeIV=O stretching band at 815 cm-1, a higher frequency than noted in any other ferrylcontaining enzyme to date. The band shows an 16O/18O frequency shift of -46 cm-1, larger than that observed for any porphyrin ferryl species. The FeIV=O formulation was unambiguously established by oxidations of the reduced enzyme with 16O2, 18O2, and 16O18O. Only the use of a mixed-isotope gas permitted discrimination between a ferryl and a peroxo structure. A catalytic cycle for the cytochrome d terminal oxidase complex is proposed, and possible reasons for the high v(Fe=O) frequency are discussed.
Original language | English (US) |
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Pages (from-to) | 11485-11489 |
Number of pages | 5 |
Journal | Biochemistry |
Volume | 30 |
Issue number | 49 |
DOIs | |
State | Published - Dec 1 1991 |
ASJC Scopus subject areas
- Biochemistry