Identification of a cytochrome bc1-aa3 supercomplex in Rhodobacter sphaeroides

Olga Fedotovskaya, Ingrid Albertsson, Gustav Nordlund, Sangjin Hong, Robert B. Gennis, Peter Brzezinski, Pia Ädelroth

Research output: Contribution to journalArticlepeer-review


Respiration is carried out by a series of membrane-bound complexes in the inner mitochondrial membrane or in the cytoplasmic membrane of bacteria. Increasing evidence shows that these complexes organize into larger supercomplexes. In this work, we identified a supercomplex composed of cytochrome (cyt.) bc1 and aa3-type cyt. c oxidase in Rhodobacter sphaeroides. We purified the supercomplex using a His-tag on either of these complexes. The results from activity assays, native and denaturing PAGE, size exclusion chromatography, electron microscopy, optical absorption spectroscopy and kinetic studies on the purified samples support the formation and coupled quinol oxidation:O2 reduction activity of the cyt. bc1-aa3 supercomplex. The potential role of the membrane-anchored cyt. cy as a component in supercomplexes was also investigated.

Original languageEnglish (US)
Article number148433
JournalBiochimica et Biophysica Acta - Bioenergetics
Issue number8
StatePublished - Aug 1 2021


  • Bioenergetics
  • Cytochrome bc complex
  • Electron transfer
  • Respiratory supercomplex
  • aa-type cytochrome c oxidase
  • flow-flash

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology


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