TY - JOUR
T1 - Identification of 9 novel transcripts and two RGSL genes within the hereditary prostate cancer region (HPC1) at 1q25
AU - Silva, Ana Paula M.
AU - Salim, Anna Christina M.
AU - Bulgarelli, Adriana
AU - De Souza, Jorge Estefano S.
AU - Osório, Elisson
AU - Caballero, Otavia L.
AU - Iseli, Christian
AU - Stevenson, Brian J.
AU - Jongeneel, C. Victor
AU - De Souza, Sandro J.
AU - Simpson, Andrew J.G.
AU - Camargo, Anamaria A.
N1 - Funding Information:
The authors thank Ricardo Pereira de Moura, Valéria Paixão, Elisângela Monteiro, Jane Kaiano, Fabiana Bettoni and Rafael Bessa Parmigiani for outstanding technical support, and Helena P. Brentani and Fabricio Falconi for critically reading this manuscript. This work was supported by Ludwig Institute for Cancer Research and Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP) through the CEPID program. APMS is supported by a fellowship from the Coordenação de Aperfeiçoamento de Pessoal de NÍvel Superior (CAPES).
PY - 2003/5/22
Y1 - 2003/5/22
N2 - We applied a systematic bioinformatics approach, followed by careful manual inspection and experimental validation to identify additional expressed sequences located at the Hereditary Prostate Cancer Region (HPC1) between D1S2818 and D1S1642 on chromosome 1q25. All transcripts already described for the 1q25 region were identified and we were able to define 11 additional expressed sequences within this region (three full-length cDNA clone sequences and eight ESTs), increasing the total number of gene count in this region by 38%. Five out of the 11 expressed sequences identified were shown to be expressed in prostate tissue and thus represent novel disease gene candidates for the HPC1 region. Here, we report a detailed characterization of these five novel disease gene candidates, their expression pattern in various tissues, their genomic organization and functional annotation. Two candidates (RGSL1 and RGSL2) correspond to novel members of the RGS family, which is involved in the regulation of G-protein signaling. RGSL1 and RGLS2 expression was detected by real-time polymerase chain reaction in normal prostate tissue, but could not be detected in prostate tumor cell lines, suggesting they might have a role in prostate cancer.
AB - We applied a systematic bioinformatics approach, followed by careful manual inspection and experimental validation to identify additional expressed sequences located at the Hereditary Prostate Cancer Region (HPC1) between D1S2818 and D1S1642 on chromosome 1q25. All transcripts already described for the 1q25 region were identified and we were able to define 11 additional expressed sequences within this region (three full-length cDNA clone sequences and eight ESTs), increasing the total number of gene count in this region by 38%. Five out of the 11 expressed sequences identified were shown to be expressed in prostate tissue and thus represent novel disease gene candidates for the HPC1 region. Here, we report a detailed characterization of these five novel disease gene candidates, their expression pattern in various tissues, their genomic organization and functional annotation. Two candidates (RGSL1 and RGSL2) correspond to novel members of the RGS family, which is involved in the regulation of G-protein signaling. RGSL1 and RGLS2 expression was detected by real-time polymerase chain reaction in normal prostate tissue, but could not be detected in prostate tumor cell lines, suggesting they might have a role in prostate cancer.
KW - Annotation
KW - Candidate gene
KW - RGS protein
KW - Transcription map
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U2 - 10.1016/S0378-1119(03)00501-8
DO - 10.1016/S0378-1119(03)00501-8
M3 - Article
C2 - 12801632
AN - SCOPUS:0037705427
SN - 0378-1119
VL - 310
SP - 49
EP - 57
JO - Gene
JF - Gene
IS - 1-2
ER -