TY - JOUR
T1 - Identification and characterization of core sludge and biofilm microbiota in anaerobic membrane bioreactors
AU - Cheng, Hong
AU - Cheng, Dan
AU - Mao, Junwen
AU - Lu, Ting
AU - Hong, Pei Ying
N1 - Publisher Copyright:
© 2019 The Authors
PY - 2019/12
Y1 - 2019/12
N2 - An analysis of sludge (i.e., 63 samples) and biofilm (i.e., 79 samples) sampled from 13 anaerobic membrane bioreactors (AnMBR) was conducted. Predominant microbial community identification and multivariate analysis indicate that these reactors showed different microbial community structure, but these differences had no impact on the overall AnMBR performance. Instead, core microbial genera which occurred in ≥90% of sludge (20 genera) and biofilm (12 genera) samples could potentially account for the AnMBR performance. A further calculation on net growth rate (NGR) of core genera in sludge suggested distribution into two main groups (i.e., I: low relative abundance and NGR, II: high relative abundance or high NGR). Consistent positive correlations between bacterial genera were observed among those that exhibited either high relative abundance or high NGR. The anaerobic microbial consortium in both sludge and biofilm were largely affected by stochastic dispersal and migration processes (i.e., neutral assembly). However, Acinetobacter spp. and Methanobacterium spp. occurred consistently in higher frequency in the biofilm but in lower occurrence frequency in the AnMBR permeate. Findings from this study suggest first, specific core microorganisms exist in the sludge regardless of the operating conditions of the AnMBRs, and second, prevention of biofoulant layer on anaerobic membranes can be devised by minimizing attachment of microbes on surfaces in a non-selective manner.
AB - An analysis of sludge (i.e., 63 samples) and biofilm (i.e., 79 samples) sampled from 13 anaerobic membrane bioreactors (AnMBR) was conducted. Predominant microbial community identification and multivariate analysis indicate that these reactors showed different microbial community structure, but these differences had no impact on the overall AnMBR performance. Instead, core microbial genera which occurred in ≥90% of sludge (20 genera) and biofilm (12 genera) samples could potentially account for the AnMBR performance. A further calculation on net growth rate (NGR) of core genera in sludge suggested distribution into two main groups (i.e., I: low relative abundance and NGR, II: high relative abundance or high NGR). Consistent positive correlations between bacterial genera were observed among those that exhibited either high relative abundance or high NGR. The anaerobic microbial consortium in both sludge and biofilm were largely affected by stochastic dispersal and migration processes (i.e., neutral assembly). However, Acinetobacter spp. and Methanobacterium spp. occurred consistently in higher frequency in the biofilm but in lower occurrence frequency in the AnMBR permeate. Findings from this study suggest first, specific core microorganisms exist in the sludge regardless of the operating conditions of the AnMBRs, and second, prevention of biofoulant layer on anaerobic membranes can be devised by minimizing attachment of microbes on surfaces in a non-selective manner.
KW - Core microorganisms
KW - Correlative interaction
KW - Net growth rate
KW - Neutral assembly
UR - http://www.scopus.com/inward/record.url?scp=85071986415&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85071986415&partnerID=8YFLogxK
U2 - 10.1016/j.envint.2019.105165
DO - 10.1016/j.envint.2019.105165
M3 - Article
C2 - 31520957
AN - SCOPUS:85071986415
SN - 0160-4120
VL - 133
JO - Environment international
JF - Environment international
M1 - 105165
ER -